Co-ordination of NDH and Cup proteins in CO2 uptake in cyanobacterium Synechocystis sp. PCC 6803.
Co-ordination of NDH and Cup proteins in CO2 uptake in cyanobacterium Synechocystis sp. PCC 6803.
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蓝藻集胞藻 PCC 6803 中 NDH 和 Cup 蛋白在 CO2 吸收中的协调
DOI：
10.1093/jxb/erx129
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发表时间：
2017-06-01
影响因子：
6.9
通讯作者：
Mi H
中科院分区：
文献类型：
--
作者：
Han X;Sun N;Xu M;Mi H
NdhD4和NdhF4对于组装含CuPB的络合物是必不可少的。NDH和CUP蛋白都参与二氧化碳吸收系统，协调二氧化碳吸收活动。在集胞藻中发现了分别含有CupA(NDH-1MS)和CupB(NDH-1MS‘)的高亲和力和低亲和力的CO2吸收系统。PCC6803，但目前尚不清楚这些复合体如何在二氧化碳吸收中发挥作用。在这项工作中，我们发现CuB的缺失显著降低了细胞的生长，并且CuPA和CuB的缺失都严重地抑制了细胞在pH 7.0以下的生长，即使在3%的二氧化碳中也是如此。光合作用放氧速率受CuPA基因缺失的影响较小，但受CuPPA基因缺失的影响较大，而CuPPA基因和CuOB基因的缺失对光合放氧速率的影响更大，尤其是对3%CO2下pH条件变化的响应。此外，我们还发现CupB在NDH-1MS‘中的组装依赖于NdhD4和NdhF4。NDH-1MS降解突变体不影响NDH-1MS‘，NDH-1MS’降解突变体不影响NDH-1MS，说明在高CO2条件下存在独立的CO2吸收系统。在ndhd缺失突变体中，光诱导的跨类囊体质膜的质子梯度被显著抑制，这表明ndhds在质子泵中发挥作用。在缺失CuPA或CuB的突变株中，碳酸氢酶活性受到部分抑制，而在同时缺失CuPA和CuB的突变株中，活性受到严重抑制，这表明CuPA和CupB在CO2转化为HCO3-的过程中起作用。反过来，Cap基因的缺失降低了跨类囊体质膜的质子梯度，而ndhds的缺失降低了CO2的水合作用。我们的结果表明，NDH-1M提供了一个碱性区域来激活参与二氧化碳吸收的CUP蛋白。
NdhD4 and NdhF4 are essential for the assembly of CupB-containing complexes. Both NDH and Cup proteins are involved in CO2-uptake systems and co-ordinate CO2 uptake activity. High and low affinity CO2-uptake systems containing CupA (NDH-1MS) and CupB (NDH-1MS′), respectively, have been identified in Synechocystis sp. PCC 6803, but it is yet unknown how the complexes function in CO2 uptake. In this work, we found that deletion of cupB significantly lowered the growth of cells, and deletion of both cupA and cupB seriously suppressed the growth below pH 7.0 even under 3% CO2. The rate of photosynthetic oxygen evolution was decreased slightly by deletion of cupA but significantly by deletion of cupB and more severely by deletion of both cupA and cupB, especially in response to changed pH conditions under 3% CO2. Furthermore, we found that assembly of CupB into NDH-1MS′ was dependent on NdhD4 and NdhF4. NDH-1MS′ was not affected in the NDH-1MS-degradation mutant and NDH-1MS was not affected in the NDH-1MS′-degradation mutants, indicating the existence of independent CO2-uptake systems under high CO2 conditions. The light-induced proton gradient across thylakoid membranes was significantly inhibited in ndhD-deletion mutants, suggesting that NdhDs functions in proton pumping. The carbonic anhydrase activity was suppressed partly in the cupA- or cupB-deletion mutant but severely in the mutant with both cupA and cupB deletion, indicating that CupA and CupB function in conversion of CO2 to HCO3–. In turn, deletion of cup genes lowered the transthylakoid membrane proton gradient and deletion of ndhDs decreased the CO2 hydration. Our results suggest that NDH-1M provides an alkaline region to activate Cup proteins involved in CO2 uptake.
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影响因子：
64.8
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
4.6
作者：
Chen X;He Z;Xu M;Peng L;Mi H
通讯作者：
Mi H
影响因子：
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作者：
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