Application of a novel fluorescence intensity assay: identification of distinct fatty acetates as volatile compounds that bind specifically to amino acid region 149-168 of a transmembrane receptor CD36
Application of a novel fluorescence intensity assay: identification of distinct fatty acetates as volatile compounds that bind specifically to amino acid region 149-168 of a transmembrane receptor CD36
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新型荧光强度测定的应用：鉴定不同的脂肪乙酸酯作为与跨膜受体 CD36 的氨基酸区域 149-168 特异性结合的挥发性化合物
DOI：
10.1093/bbb/zbac018
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Sasaki T.
中科院分区：
文献类型：
--
作者：
Tsuzuki S;Kimoto Y;Marui K;Lee S;Inoue K;Sasaki T.
分化簇36（CD 36）是在各种细胞中表达的跨膜受体，并且具有多种脂质配体。CD 36在鼠嗅上皮中的表达及其识别某些种类的脂肪醛（一类气味活性挥发性化合物）的能力表明了该受体在哺乳动物鼻腔中捕获特定气味物中的作用。然而，CD 36可识别的挥发性化合物的光谱知之甚少。在这项研究中，我们采用了我们最近设计的检测与荧光标记的肽作为探针（荧光强度测定），并确定了不同的脂肪乙酸酯作为挥发性化合物，特异性结合到CD 36的氨基酸区域149-168（如十二烷基和十四烷基乙酸酯）。目前的研究结果表明，我们的检测发现新的CD 36配体的效用和支持的概念，受体的功能作为一个捕捉器的挥发性化合物在哺乳动物的嗅觉系统。
The cluster of differentiation 36 (CD36) is a transmembrane receptor expressed in various cells and has diverse lipid ligands. The expression of CD36 in the murine olfactory epithelium and its ability to recognize certain species of fatty aldehydes, a class of odor-active volatile compounds, have suggested a role for this receptor in the capture of specific odorants in the nasal cavity of mammals. However, the spectrum of CD36-recognizable volatile compounds is poorly understood. In this study, we employed our recently devised assay with fluorescently labeled peptides as probes (fluorescence intensity assay) and identified distinct fatty acetates as volatile compounds that bind specifically to amino acid region 149-168 of CD36 (eg dodecyl and tetradecyl acetates). The present findings demonstrate the utility of our assay for the discovery of novel CD36 ligands and support the notion that the receptor functions as a captor of volatile compounds in the mammalian olfactory system.