Characterization of PC12 Cell Subclones with Different Sensitivities to Programmed Thermal Stimulation.
Characterization of PC12 Cell Subclones with Different Sensitivities to Programmed Thermal Stimulation.
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DOI：
10.3390/ijms21218356
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发表时间：
2020-11-07
影响因子：
5.6
通讯作者：
Nakai J
中科院分区：
文献类型：
--
作者：
Kudo TA;Tominami K;Izumi S;Hayashi Y;Noguchi T;Matsuzawa A;Hong G;Nakai J
神经发生是神经系统再生的基础过程，然而，促进神经元再生活动的最佳细胞外信号还需要进一步研究。在此之前，我们开发了一种新的方法，利用加热板的温控重复热刺激(TRTs)诱导大鼠PC12细胞分化。根据对TRT的神经源性敏感性，PC12细胞分为高敏感或低敏感两类。在本研究中，我们根据TRT在分化过程中的敏感性建立了两个PC12来源的亚克隆：PC12-P1F1(高敏感性亚克隆)和PC12-P1D10(低敏感性亚克隆)，以探讨低敏感性的机制。为了确定这些亚克隆的特征，对神经生长因子(NGF)、骨形态发生蛋白(BMP)或不同TRT处理的亚克隆进行了细胞大小和神经发生的评估。亲本细胞和亚克隆之间的细胞大小没有显著差异。与亲代细胞相比，BMP4或TRTS诱导的PC12-P1F1细胞神经再生增加，而PC12-P1D10细胞未观察到神经再生。相反，在所有三种细胞系中都观察到了NGF诱导的神经再生。此外，骨形态发生蛋白抑制剂LDN-193189显著抑制了TRTS诱导的神经再生。这些结果表明，BMP途径可能是TRTs诱导的神经发生所必需的，这表明了这些新的亚克隆在TRTs研究中的有用方面。
Neuritogenesis is the process underling nervous system regeneration; however, optimal extracellular signals that can promote neuronal regenerative activities require further investigation. Previously, we developed a novel method for inducing neuronal differentiation in rat PC12 cells using temperature-controlled repeated thermal stimulation (TRTS) with a heating plate. Based on neurogenic sensitivity to TRTS, PC12 cells were classified as either hyper- or hyposensitive. In this study, we aimed to investigate the mechanism of hyposensitivity by establishing two PC12-derived subclones according to TRTS sensitivity during differentiation: PC12-P1F1, a hypersensitive subclone, and PC12-P1D10, a hyposensitive subclone. To characterize these subclones, cell size and neuritogenesis were evaluated in subclones treated with nerve growth factor (NGF), bone morphogenetic protein (BMP), or various TRTS. No significant differences in cell size were observed among the parental cells and subclones. BMP4- or TRTS-induced neuritogenesis was increased in PC12-P1F1 cells compared to that in the parental cells, while no neuritogenesis was observed in PC12-P1D10 cells. In contrast, NGF-induced neuritogenesis was observed in all three cell lines. Furthermore, a BMP inhibitor, LDN-193189, considerably inhibited TRTS-induced neuritogenesis. These results suggest that the BMP pathway might be required for TRTS-induced neuritogenesis, demonstrating the useful aspects of these novel subclones for TRTS research.
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影响因子：
3.7
作者：
Kudo TA;Kanetaka H;Mochizuki K;Tominami K;Nunome S;Abe G;Kosukegawa H;Abe T;Mori H;Mori K;Takagi T;Izumi S
通讯作者：
Izumi S
影响因子：
4.4
作者：
Lönn, P;Zaia, K;Ebendal, T
通讯作者：
Ebendal, T
影响因子：
3.6
作者：
Rakhit, S;Pyne, S;Pyne, NJ
通讯作者：
Pyne, NJ
影响因子：
5.8
作者：
Kawasaki A;Okada M;Tamada A;Okuda S;Nozumi M;Ito Y;Kobayashi D;Yamasaki T;Yokoyama R;Shibata T;Nishina H;Yoshida Y;Fujii Y;Takeuchi K;Igarashi M
通讯作者：
Igarashi M
影响因子：
2.1
作者：
Kudo, Tada-aki;Kanetaka, Hiroyasu;Hayashi, Haruhide
通讯作者：
Hayashi, Haruhide