Identification of a G-protein in depolarizing rod bipolar cells.
Identification of a G-protein in depolarizing rod bipolar cells.
复制标题
去极化视杆双极细胞中 G 蛋白的鉴定。
DOI：
10.1017/s0952523800004697
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发表时间：
1993
影响因子：
1.9
通讯作者：
Sterling,P
中科院分区：
文献类型：
--
作者：
Vardi,N;Matesic,DF;Manning,DR;Liebman,PA;Sterling,P
从光感受器到去极化双极细胞的突触传递由APB谷氨酸受体介导。该受体显然与激活cGMP-磷酸二酯酶的g蛋白偶联，以调节cGMP水平，从而形成cGMP门控阳离子通道。我们试图用免疫细胞化学方法定位该系统，使用抗体来定位杆光传导级联的各种成分，包括Gt（转导蛋白）、磷酸二酯酶、cgmp门控通道和阻滞蛋白。所有这些抗体都与杆状细胞反应强烈，但没有与双极细胞反应。针对不同g蛋白Go的抗体与三种哺乳动物的棒状双极细胞（去极化且对apb敏感）发生强烈反应。同样染色的还有锥体双极细胞亚群，但不是猫的主要去极化类型（b1）。Go抗体也能染色某些蝾螈双极细胞。因此，在广泛的物种中，氧化石墨烯存在于视网膜双极细胞中，至少其中一些是去极化和apb敏感的。
Synaptic transmission from photoreceptors to depolarizing bipolar cells is mediated by the APB glutamate receptor. This receptor apparently is coupled to a G-protein which activates cGMP-phosphodiesterase to modulate cGMP levels and thus a cGMP-gated cation channel. We attempted to localize this system immunocytochemically using antibodies to various components of the rod phototransduction cascade, including Gt (transducin), phosphodiesterase, the cGMP-gated channel, and arrestin. All of these antibodies reacted strongly with rods, but none reacted with bipolar cells. Antibodies to a different G-protein, Go, reacted strongly with rod bipolar cells of three mammalian species (which are depolarizing and APB-sensitive). Also stained were subpopulations of cone bipolar cells but not the major depolarizing type in cat (b1). Go antibody also stained certain salamander bipolar cells. Thus, across a wide range of species, Go is present in retinal bipolar cells, and at least some of these are depolarizing and APB-sensitive.