DIRECT INHIBITION BY OPIOID-PEPTIDES OF NEURONS LOCATED IN THE VENTROMEDIAL NUCLEUS OF THE GUINEA-PIG HYPOTHALAMUS
DIRECT INHIBITION BY OPIOID-PEPTIDES OF NEURONS LOCATED IN THE VENTROMEDIAL NUCLEUS OF THE GUINEA-PIG HYPOTHALAMUS
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DOI：
10.1016/0006-8993(88)91551-x
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发表时间：
1988-05-31
期刊：
影响因子：
2.9
通讯作者：
DREIFUSS, JJ
中科院分区：
文献类型：
--
作者：
CHARPAK, S;DUBOISDAUPHIN, M;DREIFUSS, JJ
在豚鼠脑片上，从下丘脑腹内侧核（VMH）神经元获得细胞内记录。[D-Ala 2，NMePhe 4，Gly-ol 5]脑啡肽（DAGO），对μ-阿片受体，引起自发放电活动的抑制和膜超极化。此作用呈浓度依赖性，可逆，可被纳洛酮阻断。DAGO直接抑制VMH神经元，因为当切片灌流阻断突触传递的溶液时，它的作用持续存在。DAGO引起的超极化与膜输入电阻的显著降低有关，并且在比静息电位负30-40 mV的膜电位处极性反转。氯离子电流没有贡献DAGO所带来的超极化。我们得出结论，DAGO抑制VMH神经元，可能是通过开放膜钾通道。
In slices of guinea pig brain, intracellular recordings were obtained from neurones of the ventromedial nucleus of the hypothalamus (VMH). [D-Ala2,NMePhe4,Gly-ol5]enkephalin (DAGO), an agonist selective for .mu.-opioid receptors, caused an inhibition of spontaneous firing activity and a membrane hyperpolarization. This effect was reversible, concentration-dependent and could be blocked by naloxone. DAGO directly inhibited VMH neurones since its effect persisted when the slice was perifused with a solution that blocks synaptic transmission. The hyperpolarization induced by DAGO was associated with a marked decrease in membrane input resistance and it was reversed in polarity at membrane potentials 30-40 mV more negative than the resting potential. A chloride current did not contribute to the hyperpolarization brought about by DAGO. We conclude that DAGO inhibits VMH neurones, probably by opening membrane potassium channels.