A novel endoscopic marker: safety experiments in the rat stomach.
A novel endoscopic marker: safety experiments in the rat stomach.
复制标题
一种新型内窥镜标记物：大鼠胃中的安全性实验。
DOI：
10.1055/s-2006-925262
复制
发表时间：
2006
期刊：
影响因子：
9.3
通讯作者：
K. Saito
中科院分区：
文献类型：
--
作者：
A. Sugitachi;K. Otsuka;Y. Akiyama;T. Itabashi;H. Asahi;N. Uesugi;R. Tsutsumi;K. Saito
背景和研究目的
该研究旨在评估一种新开发的内窥镜标记物，其设计仅引起轻微的炎症反应。
材料和方法
在标记物中使用了壳聚糖和碳粉。该产品是一种粘弹性溶液，将其注射到大鼠胃壁的粘膜下层。然后对组织反应进行组织病理学检查。用电子显微镜检查大鼠胃壁中注射的标记物的结构。目前用作内窥镜标记物的印度墨水用作对照。
结果
组织病理学检查显示，在大鼠器官中，使用新型试剂的炎症反应非常轻微，而粘膜下应用印度墨水导致严重的原位炎症。电子显微镜的结果表明，所使用的碳颗粒是完全球形的形状和标记物质中的碳被截留在壳聚糖网络。印度墨水被证明是由细碳颗粒和粘合剂添加剂的混合物组成的。
结论
壳聚糖-碳溶液似乎是一种有前途的内窥镜标记物质，可显著减少炎症。
BACKGROUND AND STUDY AIMS
The study aimed to assess a newly developed endoscopic marker designed to cause only minor inflammatory reactions.
MATERIALS AND METHODS
Chitosan and carbon powder were used in the marker substance. The product was a viscoelastic solution, which was injected into the submucosa in rat stomach walls. The tissue reactions were then examined histopathologically. The structure of the injected marker substance was examined with electron microscopy into rat stomach walls. India ink, which is currently used as an endoscopic marker, served as the control.
RESULTS
Histopathological examination showed that inflammatory reactions with the novel agent were remarkably mild in the rat organs, while submucosally applied india ink caused severe inflammation in situ. The electron-microscopic findings showed that the carbon particles used were completely spherical in shape and that the carbon in the marker substance was entrapped in the chitosan networks. The india ink was shown to consist of a mixture of fine carbon particles and adhesive additives.
CONCLUSIONS
The chitosan-carbon solution appears to be a promising endoscopic marker substance, causing significantly reduced inflammation.