LOCALIZATION AND DNA-SEQUENCE ANALYSIS OF THE C-GENE OF BACTERIOPHAGE-MU, THE POSITIVE REGULATOR OF MU-LATE TRANSCRIPTION
LOCALIZATION AND DNA-SEQUENCE ANALYSIS OF THE C-GENE OF BACTERIOPHAGE-MU, THE POSITIVE REGULATOR OF MU-LATE TRANSCRIPTION
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DOI：
10.1093/nar/14.12.4881
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发表时间：
1986-06-25
影响因子：
14.9
通讯作者：
HOWE, MM
中科院分区：
文献类型：
--
作者：
MARGOLIN, W;HOWE, MM
噬菌体Mu的C基因是噬菌体晚期基因转录所必需的，通过构建和分析pKN50的一系列缺失的衍生体来定位Mu的C基因，pKN50是一个含有9.4kb的Mu DNA片段的质粒，它补充了Mu C琥珀色突变噬菌体的生长。其中一种缺失的衍生物pWMl0只含有0.5kb的Mu DNA，它补充C琥珀噬菌体并反式激活MoM基因，MoM基因是Mu晚期依赖C激活的基因之一。0.5kb片段的DNA序列预测了一个编码140个氨基酸的蛋白质的单一长开放阅读框架。对含有C琥珀章动的DNA进行的序列分析定位了该阅读框架第二密码子的碱基改变。通过填充该阅读框架的第114密码子的BkIII位点产生移码突变，导致C互补和反式激活活性的丧失。这些结果表明，该开放阅读框编码Mu C基因产物。C蛋白的预测氨基酸序列与其他转录调节蛋白的氨基酸序列比较显示，与细菌SLGMA因子中高度保守的区域有一些相似之处。
The C gene of bacteriophage Mu, required for transcription of the phage late genes, was localized by construction and analysis of a series of deleted derivatives of pKN50, a plasmid containing a 9.4 kb Mu DNA fragment which complements Mu C amber mutant phages for growth. One such deleted derivative, pWMl0, containing only 0.5 kb of Mu DNA, complements C amber phages and transactivates the mom gene, one of the Mu late genes dependent on C for activation. The DNA sequence of the 0.5 kb fragaent predicts a single long open reading frame coding for a 140 amino acid protein. Sequence analysis of DNA containing a C amber nutation located the base change to the second codon of this reading frame. Generation of a frameshift mutation by filling in a BkIII site spanning codon 114 of this reading frame resulted in the loss of C complementation and transactivation activity. These results indicate that this open reading frame encodes the Mu C gene product. Comparinon of the predicted amino acid sequence of the C protein with those of other transcriptional regulatory proteins revealed some similarity to a region highly conserved among bacterial slgma factors.