BslA(YuaB) forms a hydrophobic layer on the surface of Bacillus subtilis biofilms
BslA(YuaB) forms a hydrophobic layer on the surface of Bacillus subtilis biofilms
复制标题
DOI：
10.1111/j.1365-2958.2012.08094.x
复制
发表时间：
2012-07-01
影响因子：
3.6
通讯作者：
Iwano, Megumi
中科院分区：
文献类型：
--
作者：
Kobayashi, Kazuo;Iwano, Megumi
生物膜是表面相关的细菌聚集体，其中细菌被称为生物膜基质的聚合物包裹。枯草芽孢杆菌生物膜显示出对液体润湿和气体渗透的持久抗性，这可能解释了这些生物膜中的细菌对抗菌剂的广谱抗性。在该研究中，BslA（以前的Yua B）被鉴定为B的表面排斥性的主要贡献者。枯草杆菌生物膜破坏bslA导致表面排斥性的损失，并改变了生物膜表面的微观结构。BslA以胞外多糖依赖性方式定位于生物膜基质。纯化的BslA表现出两亲性，并形成聚合物，在体外的空气-水界面的面积的增加。遗传和生化分析表明，BslA的自聚合活性是必不可少的，其定位到生物膜基质的能力。共聚焦激光扫描显微镜显示BslA在生物膜表面形成一层。综上所述，我们认为BslA（代表生物膜表面层蛋白）负责生物膜表面的疏水层。
Biofilms are surface-associated bacterial aggregates, in which bacteria are enveloped by polymeric substances known as the biofilm matrix. Bacillus subtilis biofilms display persistent resistance to liquid wetting and gas penetration, which probably explains the broad-spectrum resistance of the bacteria in these biofilms to antimicrobial agents. In this study, BslA (formerly YuaB) was identified as a major contributor to the surface repellency of B. subtilis biofilms. Disruption of bslA resulted in the loss of surface repellency and altered the biofilm surface microstructure. BslA localized to the biofilm matrix in an exopolysaccharide-dependent manner. Purified BslA exhibited amphiphilic properties and formed polymers in response to increases in the area of the airwater interface in vitro. Genetic and biochemical analyses showed that the self-polymerization activity of BslA was essential for its ability to localize to the biofilm matrix. Confocal laser scanning microscopy showed that BslA formed a layer on the biofilm surface. Taken together, we propose that BslA, standing for biofilm-surface layer protein, is responsible for the hydrophobic layer on the surface of biofilms.