PROTEIN-LOAD EFFECTS ON THE PH GRADIENT OF ISOELECTRIC-FOCUSING IN POLYACRYLAMIDE GELS
PROTEIN-LOAD EFFECTS ON THE PH GRADIENT OF ISOELECTRIC-FOCUSING IN POLYACRYLAMIDE GELS
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DOI：
10.1016/0003-2697(81)90340-7
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发表时间：
1981-01-01
影响因子：
2.9
通讯作者：
TORRES, S
中科院分区：
文献类型：
--
作者：
CANTRELL, SJ;BABITCH, JA;TORRES, S
在聚丙烯酰胺凝胶中制备的等电聚焦梯度的最基本部分的pH值与聚焦在凝胶中的小鸡或大鼠肝脏或脑蛋白的量成正比地降低。在中等蛋白质负载量（0.3 mg）下，pH 值的降低相对较小（0.28 pH 单位），但在高蛋白质负载量下，pH 值的降低变得明显（约 1.00 pH 单位）。这种现象与两性电解质的来源无关。为了观察二维碱性蛋白质，建议将低蛋白质负载量的平衡聚丙烯酰胺凝胶与 Ag 染色相结合。
The pH of the most basic section of an isoelectric-focusing gradient prepared in a polyacrylamide gel decreases in direct proportion to the amount of chick or rat liver or brain protein focused in the gel. At modest protein loads (0.3 mg) the decrease in pH is relatively small (0.28 pH unit) but at high protein loads the decrease in pH becomes appreciable (.apprx. 1.00 pH unit). This phenomenon is independent of the source of the ampholytes. To observe basic proteins in 2-dimensional, equilibrium polyacrylamide gels low protein loads combined with Ag staining is recommended.