Regulation of human (Caco-2) intestinal epithelial cell differentiation by extracellular matrix proteins
Regulation of human (Caco-2) intestinal epithelial cell differentiation by extracellular matrix proteins
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DOI：
10.1006/excr.1996.0180
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发表时间：
1996-06-15
影响因子：
3.7
通讯作者：
Emenaker, NJ
中科院分区：
文献类型：
--
作者：
Basson, MD;Turowski, G;Emenaker, NJ
细胞外基质调节肠上皮分化可能影响发育、向绒毛尖端迁移过程中的分化、愈合、炎症性肠病和恶性转化。肠上皮生物学的细胞培养研究也可能取决于所使用的基质基质。我们评估了基质对人肠道Caco-2上皮细胞（肠上皮分化模型）分化和增殖的影响。比较了间质I型胶原和基膜成分TV型胶原和层粘连蛋白在组织培养塑料上培养的细胞的增殖、刷缘酶比活性和扩散。与塑料相比，每种基质都显著增加了碱性磷酸酶、二肽基肽酶、乳糖酶、蔗糖-异麦芽糖酶和细胞扩散。与ⅰ型胶原相比，基底膜蛋白胶原TV和层粘连蛋白对4种刷缘酶均有促进作用，但对扩散有抑制作用。ⅰ型胶原的增殖最快，而层粘连蛋白和组织培养塑料的增殖最慢。与间质性胶原相比，基底膜基质蛋白可促进肠上皮细胞分化并抑制增殖。
Extracellular matrix regulation of intestinal epithelial differentiation may affect development, differentiation during migration to villus tips, healing, inflammatory bowel disease, and malignant transformation. Cell culture studies of intestinal epithelial biology may also depend on the matrix substrate used. We evaluated matrix effects on differentiation and proliferation in human intestinal Caco-2 epithelial cells, a model for intestinal epithelial differentiation. Proliferation, brush border enzyme specific activity, and spreading were compared in cells cultured on tissue culture plastic with interstitial collagen I and the basement membrane constituents collagen TV and laminin. Each matrix significantly increased alkaline phosphatase, dipeptidyl peptidase, lactase, sucrase-isomaltase, and cell spreading in comparison to plastic. However, the basement membrane proteins collagen TV and laminin further promoted all four brush border enzymes but inhibited spreading compared to collagen I. Proliferation was most rapid on type I collagen and slowest on laminin and tissue culture plastic. Basement membrane matrix proteins may promote intestinal epithelial differentiation and inhibit proliferation compared with interstitial collagen I. (C) 1996 Academic Press, Inc.