Functional differentiation in UV-B-induced DNA damage and growth inhibition between highland and lowland ecotypes of two Arabidopsis species
Functional differentiation in UV-B-induced DNA damage and growth inhibition between highland and lowland ecotypes of two Arabidopsis species
复制标题
DOI：
10.1016/j.envexpbot.2016.07.008
复制
发表时间：
2016-11
影响因子：
5.7
通讯作者：
Qing-Wei Wang;S. Nagano;H. Ozaki;S. Morinaga;J. Hidema;K. Hikosaka
中科院分区：
文献类型：
--
作者：
Qing-Wei Wang;S. Nagano;H. Ozaki;S. Morinaga;J. Hidema;K. Hikosaka
紫外线-B（UV-B; 280 - 315 nm）辐射已被证明对海拔较高的植物更具压力。栖息在不同起源的物种可能已经进化改变了紫外线耐受性，以使其表型与当地条件相匹配。然而，人们对高地和低地之间的紫外线适应模式知之甚少。本研究以不同海拔的两种不同生态类型的紫茎泽兰、四种不同生态类型的紫茎泽兰为研究对象，研究了紫外线对紫茎泽兰DNA的损伤和紫茎泽兰生长的影响。thaliana和A.哈勒亚种在补充UV-B下萌发。在UV-B处理前和暴露于增强的UV-B照射后的早期和晚期进行收获。测定环丁烷嘧啶二聚体（CPD）的积累水平作为UV对DNA损伤的量度。在生长初期，两种低地生态类型均表现出较高的CPD水平和对生物量生产的抑制作用，表明低地生态类型比高地生态类型对UV-B的增加更为敏感。相反，在生长后期，低地生态类型的CPD水平和生长抑制变得相似甚至更低。这些结果表明，紫外线胁迫的反应是组成的高原生态型，但更诱导在低地生态型。相对生长率与CPD水平呈负相关。这些生态型分化是共同的twoadopsisspecies，表明当地的适应发生在平行的生长和紫外线耐受性之间的权衡的约束下。
Ultraviolet-B (UV-B; 280–315 nm) radiation has been shown to be more stressful for plants at higher elevations. Species inhabiting different origins may have evolutionarily altered UV tolerance to match their phenotypes to local conditions. However, little is known about UV adaptation patterns between high- and lowland. Here, we evaluated UV damage to DNA and growth in ecotypes of two species ofArabidopsisfrom different elevations, four ecotypes ofA. thalianaand three ecotypes ofA. hallerisubsp. gemmiferaunder supplemental UV-B. Harvests were done before UV-B treatment and at early and late stages after the exposure to enhanced UV-B irradiation. The accumulation level of cyclobutane pyrimidine dimer (CPD) was determined as a measure of UV damage to DNA. At the early stage, lowland ecotypes of two species exhibited a higher CPD level and greater inhibition in biomass production, indicating that lowland ecotypes were more sensitive to increased UV-B than highland ecotypes. In contrast, at the later stage, CPD level and growth inhibition became similar or even lower in lowland ecotypes. These results suggest that the response to UV stress was constitutive in highland ecotypes but more inducible in lowland ecotypes. The relative growth rate was negatively related to CPD level. These ecotypic differentiations were common to twoArabidopsisspecies, suggesting that local adaptation occurred in parallel under a constraint of tradeoff between growth and UV tolerance.