ndependent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription
ndependent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription
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DOI：
10.7554/elife.30178.001
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Jin-Qiu Zhou
中科院分区：
文献类型：
--
作者：
Zhen Zhou;Yu-Ting Liu;Li Ma;Ting Gong;Ya-Nan Hu;Hong-Tao Li;Chen Cai;Ling-Li Zhang;Gang Wei;Jin-Qiu Zhou
Histone tail modifications can greatly influence chromatin-associated processes. Asymmetrically modified nucleosomes exist in multiple cell types, but whether modifications on both sister histones contribute equally to chromatin dynamics remains elusive. Here, we devised a bivalent nucleosome system that allowed for the constitutive assembly of asymmetrically modified sister histone H3s in nucleosomes in Saccharomyces cerevisiae. The sister H3K36 methylations independently affected cryptic transcription in gene coding regions, whereas sister H3K79 methylation had cooperative effects on gene silencing near telomeres. H3K4 methylation on sister histones played an independent role in suppressing the recruitment of Gal4 activator to the GAL1 promoter and in inhibiting GAL1 transcription. Under starvation stress, sister H3K4 methylations acted cooperatively, independently or redundantly to regulate transcription. Thus, we provide a unique tool for comparing symmetrical and asymmetrical modifications of sister histone H3s in vivo.
