EXTENSIVE CORTICAL DEPTH MEASUREMENTS AND NEURON SIZE INCREASES IN CORTEX OF ENVIRONMENTALLY ENRICHED RATS
EXTENSIVE CORTICAL DEPTH MEASUREMENTS AND NEURON SIZE INCREASES IN CORTEX OF ENVIRONMENTALLY ENRICHED RATS
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DOI：
10.1002/cne.901310305
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发表时间：
1967-01-01
影响因子：
2.5
通讯作者：
RAYMOND, A
中科院分区：
文献类型：
--
作者：
DIAMOND, MC;LINDNER, B;RAYMOND, A
这项研究是一系列调查中的另一项，试图将大脑的变化定义为环境操纵的结果。皮质深度和神经元大小的测量采取了丰富的（ECT）和贫困（IC）的环境中的大鼠的大脑。用硫堇对福尔马林固定的大鼠脑的火棉胶包埋的10 [mu]横切面进行染色。从每个大脑中取10个代表性切片，利用皮质下标志以确保均匀取样。从中线开始并向侧面进行，在显微玻片投影图上测量皮质深度。每个半球的读数范围为41至46。在后连合标志的切片中发现深度增加最大。在这个位置，从内侧到外侧，4个区域的增加如下：A，5.2%（p< 0.001）; B，6.3%（p < 0.001）; C，3.2%（p< 0.01）; D，1.6%（NS）。用求积仪在缩微胶片投影轮廓上进行核周体和核大小测量。皮质分为上、中、下三层，在每三分之一层内，注意到细胞大小增加，外三分之一显示出最大的增加。上、中、下节段ECT细胞核比IC细胞核分别大17.7%（p < 0.001）、7.2%（p< 0.05）和11.6%（p< 0.001）。
This study is another in a series of investigations attempting to define the changes in the brain as a consequence of environmental manipulation. Cortical depth and neuron size measurements were taken on brains of rats from enriched (ECT) and impoverished (IC) environments. Celloidin embedded, 10 [mu], transverse sections of formalin fixed rat brains were stained with thionin. Ten representative sections from each brain were taken, utilizing subcortical landmarks to insure uniform sampling. Cortical depths were measured on microslide projected drawings beginning at the mid-line and proceeding laterally. The number of readings per hemisphere ranged from 41 to 46. The greatest increases in depth were found in the sections from the posterior commissure landmark. At this position the increases in 4 regions, proceeding medially to laterally, were as follows: A, 5.2% (p< 0.001); B, 6.3% (p < 0.001); C, 3.2% (p< 0.01); D, 1.6% (NS). Perikarya and nuclear size measurements were taken with a planimeter on microfilm projected outlines. The cortex was divided into upper, middle and lower levels, and within each third, cell size increases were noted, with the outer third showing the greatest increases. The ECT perikarya were greater than the IC by 17.7% (p < 0.001) in the upper level, by 7.2% (p< 0.05) in the middle level, and by 11.6% (p< 0.001) in the lower level.