Preference of the recombination sites involved in the formation of extrachromosomal copies of the human alphoid Sau3A repeat family.
Preference of the recombination sites involved in the formation of extrachromosomal copies of the human alphoid Sau3A repeat family.
复制标题
参与人类 alphoid Sau3A 重复家族染色体外拷贝形成的重组位点的偏好。
DOI：
10.1093/nar/23.24.4971
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发表时间：
1995
影响因子：
14.9
通讯作者：
R. Kiyama
中科院分区：
文献类型：
--
作者：
R. Ohki;M. Oishi;R. Kiyama
人α-Sau 3A重复家族DNA是在几种细胞系中被主动扩增以形成染色体外环状DNA的DNA种类之一。环化发生在五个约170 bp亚基中的两个之间，平均同源性为73.1%，以及相同的亚基之间。为了研究重组反应的性质，我们克隆并分析了含有重组接头的亚基。对68个连接点的分析表明，重组优先发生在170 bp亚基结构中的4个位置10-25（A）、40-50（B）、85-90（C）和135-160（D）。两个区域（B和C）与亚基之间具有较高同源性的区域重叠，而另外两个区域（A和D）不能仅用亚基之间的区域同源性来解释。这些区域位于核小体和接头区域的两个连接处，并且与α蛋白和CENP-B的结合基序重叠。约90%的重组发生在位于下一个亚基之间（+/-2移位），尽管相邻亚基之间的重组频率（+/-1移位）约为10%。
The human alphoid Sau3A repetitive family DNA is one of the DNA species that are actively amplified to form extrachromosomal circular DNA in several cell lines. The circularization takes place between two of the five approximately 170 bp subunits with an average of 73.1% homology as well as between identical subunits. To investigate the nature of the recombination reaction, we cloned and analyzed the subunits containing recombination junctions. Analysis of a total of 68 junctions revealed that recombination had occurred preferentially at four positions 10-25 (A), 40-50 (B), 85-90 (C) and 135-160 (D) in the 170bp subunit structure. Two regions (B and C) were overlapped with the regions with higher homology between subunits, while other two regions (A and D) cannot be explained solely by the regional homology between the subunits. These regions were located at both junctions of the nucleosomal and the linker region, and overlapped with the binding motifs for alpha protein and CENP-B. Approximately 90% of the recombination occurred between the subunits located next but one (+/- 2 shift), although the frequency of recombination between the adjoining subunits (+/- 1 shift) was approximately 10%.