Recovery and identification of West Nile virus from a hawk in winter
Recovery and identification of West Nile virus from a hawk in winter
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DOI：
10.1128/jcm.38.8.3110-3111.2000
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发表时间：
2000-08-01
影响因子：
9.4
通讯作者：
West, AB
中科院分区：
文献类型：
--
作者：
Garmendia, AE;Van Kruiningen, HJ;West, AB
西尼罗河病毒是从 2000 年 2 月在纽约州韦斯特切斯特县死亡的一只红尾鹰的大脑中回收的，在大脑中观察到了多个神经胶质细胞、淋巴细胞和一些固缩核的病灶。用脑匀浆接种 Vero 细胞后 3 至 4 天，检测到细胞病变。分别通过荧光抗体测试或酶联免疫吸附测定揭示固定细胞或细胞裂解物中西尼罗病毒抗原的存在。此外，使用西尼罗河病毒 NS3 基因特异性引物的逆转录酶 PCR 产生了预期大小（470 bp）的扩增子，受感染的 Vero 细胞薄片的电子显微镜显示，细胞质囊泡内存在直径约 40 nm 的病毒颗粒。在蚊子不再活跃很久之后的隆冬，西尼罗河病毒感染的证明具有重要意义，因为它证明了病毒在蚊子季节之后的地区仍然存在，并暗示了另一种传播途径：在这种情况下，是捕食者的猎物。
West Nile virus was recovered from the brain of a red-tailed hawk that died in Westchester County, N.Y., in February 2000, Multiple foci of glial cells, lymphocytes, and a few pyknotic nuclei were observed in the brain. Three to 4 days after inoculation of Vero cells with brain homogenates, cytopathic changes were detected. The presence of West Nile virus antigen in fixed cells or cell lysates was revealed by fluorescent antibody testing or enzyme-linked immunosorbent assay, respectively. Furthermore, Reverse transcriptase-PCR with primers specific for the NS3 gene of West Nile virus resulted in an amplicon of the expected size (470 bp), Electron microscopy of thin sections of infected Vero cells revealed the presence of viral particles approximately 40 nm in diameter, within cytoplasmic vesicles. The demonstration of infection with the West Nile virus in the dead of the winter, long after mosquitoes ceased to be active, is significant in that it testifies to the survival of the virus in the region beyond mosquito season and suggests another route of transmission: in this case, prey to predator.