Interleukin-8, a promising predictor for prognosis of pancreatic cancer
Interleukin-8, a promising predictor for prognosis of pancreatic cancer
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Interleukin-8，一种有前景的胰腺癌预后预测因子。
DOI：
10.3748/wjg.v18.i10.1123
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发表时间：
2012-03-14
影响因子：
4.3
通讯作者：
Zhu, Ming-Hua
中科院分区：
文献类型：
--
作者：
Chen, Ying;Shi, Min;Zhu, Ming-Hua
目的：探讨促炎趋化因子白细胞介素-8 （IL-8）对胰腺癌预后的预测价值。方法：采用免疫组化方法检测胰腺癌和慢性胰腺炎组织中IL-8及其受体CXCR1的表达。采用酶联免疫吸附法检测胰腺癌患者血清IL-8水平。将人胰腺癌组织异位移植到免疫缺陷小鼠体内，观察血清IL-8对肿瘤发生的影响。结果：IL-8和CXCR1蛋白在胰腺腺癌组织（分别为55.6%和65.4%）和匹配的癌旁组织（分别为25.9%和12.3%,P < 0.01）及慢性胰腺炎（分别为0%和25%，P < 0.05）中均有过表达。胰腺癌患者血清IL-8水平（271.1 +/- 187.7 ng/mL）高于胃癌（41.77 +/- 9.11 ng/mL, P = 0.025）、结直肠癌（78.72 +/- 80.60 ng/mL, P = 0.032）、肝癌（59.60 +/- 19.80 ng/mL, P = 0.016）等消化系统肿瘤。体内肿瘤发生分析进一步证明，血清IL-8水平较高的患者肿瘤组织生长速度快于IL-8水平较低的患者。结论：IL-8可作为预测胰腺癌预后的良好血清标志物。(c) 2012年白石登。版权所有。
AIM: To investigate the value of interleukin-8 (IL-8), a pro-inflammatory chemokine, in predicting the prognosis of pancreatic cancer.METHODS: Expression of IL-8 and its receptor CXCR1 was assessed by immunohistochemistry in pancreatic cancer and chronic pancreatitis samples. Enzyme-linked immunosorbent assay was used to detect the serum IL-8 levels in pancreatic cancer patients. Human pancreatic cancer tissues were heterotopically transplanted to the immune-deficiency mice to evaluate the effect of serum IL-8 on the tumorigenesis of the cancer samples.RESULTS: IL-8 and CXCR1 proteins were both overexpressed in pancreatic adenocarcinoma samples (55.6% and 65.4%, respectively) compared with the matched para-cancer tissues (25.9% and 12.3%, P < 0.01), or chronic pancreatitis (0% and 25%, P < 0.05). Serum IL-8 levels in pancreatic cancer patients (271.1 +/- 187.7 ng/mL) were higher than in other digestive system tumors, such as gastric cancer (41.77 +/- 9.11 ng/mL, P = 0.025), colorectal carcinoma (78.72 +/- 80.60 ng/mL, P = 0.032) and hepatocellular carcinoma (59.60 +/- 19.80 ng/mL, P = 0.016). In vivo tumorigenesis analysis further proved that tumor tissues from patients with higher serum IL-8 levels grew faster than those with lower IL-8 levels.CONCLUSION: IL-8 can be a fine serum marker for predicting the prognosis pancreatic cancer. (c) 2012 Baishideng. All rights reserved.