Does diamide treatment of intact human erythrocytes cause a loss of phospholipid asymmetry?
Does diamide treatment of intact human erythrocytes cause a loss of phospholipid asymmetry?
复制标题
二酰胺处理完整的人红细胞是否会导致磷脂不对称性丧失？
DOI：
10.1016/0005-2736(86)90106-9
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
L. van Deenen
中科院分区：
文献类型：
--
作者：
P. Franck;J. Op den Kamp;B. Roelofsen;L. van Deenen
Diamide-treated human erythrocytes have been compared with native red cells as to the accessibility of their amino phospholipids to both phospholipase A2hydrolysis and fluorescamine labeling. In agreement with observations by others (Haest, C.W.M., Plasa, G., Kamp, D. and Deuticke, B. (1978) Biochim. Biophys. Acta509, 21–32), treatment of intact human erythrocytes with diamide resulted in considerably enhanced degradation of amino phospholipids upon subsequent incubation of the cells with bee venom phospholipase A2. The hydrolysis of phosphatidylethanolamine (PE) in control cells reached a plateau value at 5% after 10 min. In diamide-treated cells, on the other hand, PE hydrolysis did not level off. Contrastingly, dose-response curves recorded for the labeling of PE with the very fast reacting NH2-group-specific reagent, fluorescamine, showed identical results for both native and diamide-treated erythrocytes. In each of these two cases, a plateau was reached after approx. 15% of the PE had been labeled. These results strongly suggest that the enhanced phospholipase-A2-induced hydrolysis of amino phospholipids in diamide-treated erythrocytes may reflect a destabilization of the lipid bilayer, rather than anin situloss of phospholipid asymmetry.
DOI：
--
发表时间：
1982-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
N. Mohandas;J. Wyatt;S. Mel;M. Rossi;S. Shohet
通讯作者：
N. Mohandas;J. Wyatt;S. Mel;M. Rossi;S. Shohet
遗传性焦磷酸细胞增多症中磷脂酰胆碱的异常跨双层流动性反映了膜骨架的热敏感性增加。
DOI：
10.1016/0005-2736(85)90296-2
发表时间：
1985
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Franck,PF;OpdenKamp,JA;Lubin,B;Berendsen,W;Joosten,P;Briët,E;vanDeenen,LL;Roelofsen,B
通讯作者：
Roelofsen,B