CHANGES IN CELLULAR-LEVELS OF ATP AND ITS CATABOLITES IN ISCHEMIC RAT-LIVER
CHANGES IN CELLULAR-LEVELS OF ATP AND ITS CATABOLITES IN ISCHEMIC RAT-LIVER
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DOI：
10.1093/oxfordjournals.jbchem.a133822
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发表时间：
1982-01-01
影响因子：
2.7
通讯作者：
KAWASHIMA, Y
中科院分区：
文献类型：
--
作者：
KAMIIKE, W;WATANABE, F;KAWASHIMA, Y
采用高压液相色谱法，以理论塔板数测定了缺血大鼠肝细胞内腺嘌呤核苷酸及其代谢产物的含量。该方法足够灵敏，可以测量约1 mg组织中的所有代谢物，并检查缺血时单个肝脏中代谢物水平的变化。缺血时细胞内ATP水平迅速下降。同时，AMP短暂增加，随后通过腺苷降解为尿囊素，并蓄积所有种类的嘌呤catenin。NAD也逐渐降解，伴随着烟酰胺的蓄积。总腺嘌呤核苷酸的水平在缺血期间下降，这种减少的量等于产生的catenorase的量的总和。缺血时间< 15 min后，ATP水平在再循环时迅速恢复。ATP水平的恢复受到长时间缺血的抑制，在缺血2 h后未观察到。在缺血中积累的中间嘌呤catenate也清除再循环过程中，无论是通过它们在血流中的去除或通过进一步的氧化降解，但他们没有抢救重新使用，直到细胞水平的ATP恢复。别嘌呤醇给药导致次黄嘌呤在缺血肝脏中显著蓄积，但这种药物和氯丙嗪对再循环期间ATP水平的恢复均无明显影响。
The cellular levels of adenine nucleotides and their metabolites in ischemic rat liver were assayed by high pressure liquid chromatography with high theoretical plate numbers. The method was sensitive enough to measure all the metabolites in about 1 mg of tissue, and to examine changes in their levels in a single liver in ischemia. In ischemia the cellular level of ATP decreased rapidly. Concomitantly there was a transitory increase in AMP, followed by its degradation to allantoin via adenosine with accumulation of all species of purine catabolites. NAD was also degraded gradually with concomitant accumulation of nicotinamide. The level of total adenine nucleotides decreased during ischemia; the amount of this decrease was equal to the sum of the amounts of catabolites produced. The ATP level was rapidly restored on recirculation after an ischemic period lasting < 15 min. Recovery of the ATP level was depressed by prolonged ischemia and was not observed after an ischemic period of 2 h. Intermediate purine catabolites that accumulated in ischemia were also cleared during recirculation either by their removal in the blood flow or by further oxidative degradation, but they were not salvaged for reuse until the cellular level of ATP was restored. Administration of allopurinol resulted in marked accumulation of hypoxanthine in ischemic liver, but neither this drug nor chlorpromazine had any appreciable effect on recovery of the ATP level during recirculation.