Ontogenetic changes of proteins of endoplasmic reticulum.
Ontogenetic changes of proteins of endoplasmic reticulum.
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内质网的蛋白质的个体发生变化。
DOI：
10.1083/jcb.52.3.733
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发表时间：
1972-03
影响因子：
7.8
通讯作者：
Bresnick, E
中科院分区：
文献类型：
--
作者：
Black, O Jr;Bresnick, E
用凝胶电泳法比较了两种放射性标记的前体（14c标记的氨基酸和δ-氨基乙酰丙酸- 3h）掺入胎、未成熟和成年雄性大鼠的光滑内质网和粗糙内质网的蛋白质。标记模式表明，存在于两个主要电泳带中的蛋白质成分在胎儿组织中进行了显著的合成，而在成人组织中则注意到三个积极结合的蛋白质带。虽然在肝脏发育过程中，整个内质网的光滑和粗糙成分对氨基酸- 14c的吸收减少，但在成人和胎儿肝脏中，定性模式没有显著差异。血红素前体进入光滑和粗糙成分的总体掺入量（每分钟每毫克蛋白质的崩解量）也不随发育而改变。然而，随着发育，分布电泳模式发生了变化。对分子量的估计（通过圆盘电泳）和血红素前体的掺入表明，这两条主要蛋白带与内质网的细胞色素P-450和细胞色素b5相似，细胞色素b5被认为参与了混合功能氧化酶系统。有证据表明，在氧化酶能力较低的胎儿肝脏中，这两种蛋白质组的氨基酸掺入量与成人肝脏相同。然而，血红素片段的掺入在发育过程中有所不同，在细胞色素b5区减少，在细胞色素P-450区增加。这些结果与肝脏发育过程中氧化酶活性的增加有关。
The proteins of the smooth and rough endoplasmic reticulum from fetal, immature, and adult male rats were compared after incorporation of two radioactively labeled precursors, 14C-labeled amino acids and δ-aminolevulinic acid-3H by means of gel electrophoresis. The labeling patterns indicated that protein components present in two major electrophoretic bands underwent significant synthesis in fetal tissue while three actively incorporating protein bands were noted in adult tissue. Although the uptake of the amino acids-14C decreased for the smooth and rough elements of the endoplasmic reticulum as a whole during liver development, the qualitative patterns were not significantly different in adult and fetal livers. The over-all incorporation (disintegrations per minute per milligram protein) of the heme precursor into the smooth and rough elements also did not change with development. However, a change was noted in the distributional electrophoretic patterns with development. The estimation of molecular weight (by disc electrophoresis) and the incorporation of the heme precursor suggested the similarity of the two major protein bands to cytochrome P-450 and cytochrome b 5, components of the endoplasmic reticulum, thought to be involved in the mixed-function oxidase system. The evidence indicated that in fetal liver, at a time when the oxidase capability was low, the amino acid incorporation into these two protein groups was the same as in the adult. The incorporation of the heme moiety, however, was different, decreasing in the cytochrome b 5 region and increasing in the cytochrome P-450 region during development. These results correlate with the increase in oxidase activity associated with liver development.