Leukotriene B4 is a potent and stereospecific stimulator of neutrophil chemotaxis and adherence.
Leukotriene B4 is a potent and stereospecific stimulator of neutrophil chemotaxis and adherence.
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Leukotriene B4 是中性粒细胞趋化性和粘附性的有效立体特异性刺激剂。
作者：
J. Palmblad;C. Malmsten;A. Udén;O. Rådmark;L. Engstedt;B. Samuelsson
我们研究了白三烯对中性粒细胞（PMN）功能的影响。在最佳浓度为10（-6）M的琼脂糖下，白细胞三烯B4（LTB 4）诱发中性粒细胞的刺激和定向迁移，而两种非酶促形成的异构体（化合物I和II）在10（-5）M时诱导了这种反应。白三烯C4（LTC 4）和5-羟基二十碳四烯酸（5-HETE）不影响PMN的迁移。在相同的最佳浓度下，LTB 4和化合物I和II增强了PMN对尼龙纤维的粘附。在10（-7）M时，趋化和粘附反应与formal-Met-Leu-Phe（fMLP）相同。没有一种白三烯影响自发或吞噬相关的化学发光或杀死金黄色葡萄球菌的能力。环氧合酶抑制剂吲哚美辛在高浓度时仅部分抑制fMLP诱导的迁移，在2.5 × 10 - 7 M时刺激迁移，表明花生四烯酸主要通过脂氧合酶途径代谢。脂氧合酶和环氧合酶抑制剂二十碳四炔酸在大于或等于2.5 × 10 - 5 M时抑制自发和刺激迁移，但在较低浓度时不抑制。因此，由于LTB 4以及在较小程度上化合物I和II刺激迁移和粘附，因此表明这些介质对于嗜中性粒细胞从血管迁移到炎症区域可能是重要的。
We studied the effects of leukotrienes on in vitro functions of neutrophil polymorphonuclear (PMN) granulocytes. Leukotriene B4 (LTB4) evoked a stimulated and directed migration of neutrophils under agarose with an optimum concentration of 10(-6)M, whereas two nonenzymatically formed isomers (compounds I and II) induced this response at 10(-5)M. Leukotriene C4 (LTC4) and 5-hydroxyeicosate-traenoic acid (5-HETE) did not affect this PMN migration. At the same optimum concentrations, LTB4 and compounds I and II augmented PMN adherence to nylon fibers. The chemotactic and adherence responses were of the same magnitude as with formal-Met-Leu-Phe (fMLP) at 10(-7)M. None of the leukotrienes influenced the spontaneous or phagocytosis-associated chemiluminescence or the ability to kill Staphylococcus aures. The cyclooxygenase inhibitor, indomethacin, inhibited only partly the fMLP-induced migration at high concentrations and stimulated migration at 2.5 x 10(-7)M, suggesting that arachidonic acid was then mainly metabolized by the lipoxygenase pathways. The lipoxygenase and cyclooxygenase inhibitor, eicosatetraynoic acid, inhibited both spontaneous and stimulated migration at greater or equal to 2.5 x 10(-5)M, but not at lower concentrations. Thus, since LTB4, and to a lesser degree compounds I and II, stimulated migration and adhesion, it is suggested that these mediators could be of importance for the emigration of neutrophils from blood vessels to areas of inflammation.
中性粒细胞的“分泌代码”。
影响因子：
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作者：
Smolen,JE;Korchak,HM;Weissmann,G
通讯作者：
Weissmann,G