Detoxification of furfural in Corynebacterium glutamicum under aerobic and anaerobic conditions
Detoxification of furfural in Corynebacterium glutamicum under aerobic and anaerobic conditions
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DOI：
10.1007/s00253-014-5924-z
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发表时间：
2014-10-01
影响因子：
5
通讯作者：
Kondo, Akihiko
中科院分区：
文献类型：
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作者：
Tsuge, Yota;Hori, Yoshimi;Kondo, Akihiko
木质纤维素水解物中的有毒发酵抑制剂对燃料和化学品的微生物生产提出了严重的问题。糠醛被认为是这些抑制剂中毒性最大的化合物之一。在这里，我们描述了糠醛在谷氨酸棒杆菌ATCC 13032在有氧和厌氧条件下的解毒。在好氧培养条件下，糠醛的脱毒产物为糠醇和2-糠酸。产物的比率根据初始糠醛浓度而变化。糠醇和2-糠酸对细胞生长都没有毒性作用，这两种化合物被确定为糠醛降解的最终产物。有趣的是，与有氧条件下不同，大部分糠醛在厌氧条件下转化为糠醇，而不影响葡萄糖消耗速率。在好氧和厌氧条件下，随着糠醛浓度的增加，NADH/NAD（+）和NADPH/NADP（+）的比值均呈下降趋势。这些结果表明，在C.谷氨酸ATCC 13032。
The toxic fermentation inhibitors in lignocellulosic hydrolysates raise serious problems for the microbial production of fuels and chemicals. Furfural is considered to be one of the most toxic compounds among these inhibitors. Here, we describe the detoxification of furfural in Corynebacterium glutamicum ATCC13032 under both aerobic and anaerobic conditions. Under aerobic culture conditions, furfuryl alcohol and 2-furoic acid were produced as detoxification products of furfural. The ratio of the products varied depending on the initial furfural concentration. Neither furfuryl alcohol nor 2-furoic acid showed any toxic effect on cell growth, and both compounds were determined to be the end products of furfural degradation. Interestingly, unlike under aerobic conditions, most of the furfural was converted to furfuryl alcohol under anaerobic conditions, without affecting the glucose consumption rate. Both the NADH/NAD(+) and NADPH/NADP(+) ratio decreased in the accordance with furfural concentration under both aerobic and anaerobic conditions. These results indicate the presence of a single or multiple endogenous enzymes with broad and high affinity for furfural and co-factors in C. glutamicum ATCC13032.