Polyclonal antibodies to phospholipid/Ca2+-dependent protein kinase and immunocytochemical localization of the enzyme in rat brain.
Polyclonal antibodies to phospholipid/Ca2+-dependent protein kinase and immunocytochemical localization of the enzyme in rat brain.
复制标题
磷脂/Ca2 依赖性蛋白激酶的多克隆抗体以及该酶在大鼠脑中的免疫细胞化学定位。
DOI：
10.1073/pnas.82.9.3030
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发表时间：
1985
影响因子：
11.1
通讯作者：
Kuo,JF
中科院分区：
文献类型：
--
作者：
Girard,PR;Mazzei,GJ;Wood,JG;Kuo,JF
抗磷脂/钙离子依赖性蛋白激酶（蛋白激酶C）的抗血清在兔中提出。通过免疫印迹和ELISA确定的多克隆抗体的免疫特异性通过其对酶的反应性而不是对其他蛋白激酶或许多其他测试蛋白中的任何一种的反应性来显示。在大鼠大脑中的激酶的免疫细胞化学定位显示，虽然酶广泛分布在不同的大脑区域，它是高度限制在大脑皮层的神经元的细胞核的周边和轴突和细胞强烈类似少突胶质细胞在白质区域。最初的大脑皮层的电子显微镜显示，该酶是高度集中在突触前的终端，只有很少被标记的突触后专业化元素看到。有人建议，离散的本地化的酶，这是不同的钙调蛋白/Ca 2+依赖的系统，可能与某些生物和功能方面的大脑，由Ca 2+调节的蛋白磷酸化的水平。
Antisera against phospholipid/Ca2+-dependent protein kinase (protein kinase C) were raised in rabbits. Immunospecificity of the polyclonal antibodies, as determined by immunoblot and ELISA, was shown by their reactivity to the enzyme but not to other protein kinases or any of many other proteins tested. Immunocytochemical localization of the kinase in rat brains revealed that although the enzyme was distributed broadly in different brain regions, it was highly restricted to the periphery of the nucleus of neurons in cerebral cortex and to axons and cells strongly resembling oligodendroglia in white-matter regions. Initial electron microscopy of cerebral cortex revealed that the enzyme was highly concentrated in the presynaptic terminals, and only rarely were labeled postsynaptic specialization elements seen. It is suggested that the discrete localization of the enzyme, which is distinct from that of the calmodulin/Ca2+-dependent system, may be related to certain biological and functional aspects of brain that are regulated by Ca2+ at the level of protein phosphorylation.