The interaction of acridine dyes with DNA: an x-ray diffraction and optical investigation.
The interaction of acridine dyes with DNA: an x-ray diffraction and optical investigation.
复制标题
吖啶染料与 DNA 的相互作用：X 射线衍射和光学研究。
DOI：
10.1016/s0022-2836(66)80094-3
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发表时间：
1966
影响因子：
5.6
通讯作者：
David R. Davies
中科院分区：
文献类型：
--
作者：
David M. Neville;David R. Davies
DNA 与吖啶橙和丙黄素的相互作用已通过 X 射线衍射和光学技术进行了研究。每三个碱基对含有一个染料分子的复合物的纤维可以在其衍射图案与钠-DNA的B型图案非常相似的条件下观察到。在较高的相对湿度下，这些纤维衍射图案变得更加无序，并且显示出前三层线的层线间距增加。通过使用光学变换已经证明，这些结果在质量上与 Lerman (1961) 的插层假设一致，尽管考虑了另一种模型也可以解释衍射图案的变化，但不能解释其他研究人员的结果 (Luzzati, Masson & Lerman, 1961; Cairns, 1962)。这些染料-DNA复合物的溶液和薄膜的吸收光谱的测量已结合X射线衍射测量进行了解释，并证明并非所有结合的染料都被嵌入，相当大的部分被结合在外部。润湿纤维时发生的吸收光谱和层线间距的变化表明染料在外部和嵌入位点之间的分布是纤维水合的函数。与非平面三苯甲烷染料对玫瑰苯胺的比较研究未能揭示插层的层线变化特征。
The interaction of DNA with acridine orange and with proflavine has been investigated by X-ray diffraction and optical techniques. Fibers of the complexes containing one dye molecule per three base pairs can be observed under conditions where their diffraction patterns closely resemble theB-form pattern of sodium-DNA. At higher relative humidities these fiber diffraction patterns become more disordered and show an increase in the layer-line spacing of the first three layer lines. It has been demonstrated by the use of optical transforms that these results are qualitatively consistent with the intercalation hypothesis of Lerman (1961), although one other model is considered which can also account for the changes in the diffraction pattern, but not for the results of other investigators (Luzzati, Masson & Lerman, 1961; Cairns, 1962). Measurements of the absorption spectra of solutions and of films of these dye-DNA complexes have been interpreted in conjunction with the X-ray diffraction measurements, and demonstrate that not all of the bound dye is intercalated, an appreciable fraction being bound on the outside. The changes in the absorption spectra and in the layer-line spacings which occur upon wetting the fiber show that the distribution of dye between the external and intercalated site is a function of the hydration of the fiber. A comparative study with the non-planar triphenylmethane dye,p-rosaniline, failed to reveal layer-line changes characteristic of intercalation.