Formation of microtubules at low temperature by tubulin from antarctic fish.
Formation of microtubules at low temperature by tubulin from antarctic fish.
复制标题
南极鱼微管蛋白在低温下形成微管。
DOI：
10.1021/bi00332a029
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
DeVries,AL
中科院分区：
文献类型：
--
作者：
WilliamsJr,RC;Correia,JJ;DeVries,AL
Department of Physiology and Biophysics，University of Illinois，Urbana，Illinois 61801 Received September 5，1984; Revised Mandarin pt Received December 10，1984摘要：通过温度依赖性组装、离心、拆卸和离心的循环，从两种无脊椎鱼类Pagothenia borchgrevinki和Dissostichus mawsoni中分离出微管蛋白。这些制剂被发现几乎完全由微管蛋白组成，并含有可忽略不计的少量微管相关蛋白。微管蛋白聚合后形成普通尺寸的微管。形成的微管似乎在所有温度下都与游离微管蛋白二聚体处于不稳定平衡。在由0.1 M 1，4-哌嗪二乙磺酸、2 mM二硫代硫酸钠、1 mM MgS 〇 4、2 mM乙二醇双（3-氨基乙基醚）-N，N，N-四乙酸和ImM鸟苷5 '-三磷酸，pH 6.9，在35 ℃下，P. borchgrevinki的微管蛋白具有0.046（± 0.008）mg/mL的组装临界浓度，在鱼的栖息地温度（-1.8 ℃）下，微管蛋白具有0.74（±0.15）mg/mL的组装临界浓度。在较低温度下测得的（deal）浓度相对于临界浓度相当小
Department of Physiology and Biophysics, University of Illinois, Urbana, Illinois 61801 Received September 5, 1984; Revised Manuscript Received December 10, 1984 abstract: Tubulin was isolated from two species of antarctic fish, Pagothenia borchgrevinki and Dissostichus mawsoni, by cycles of temperature-dependent assembly, centrifugation, disassembly, and centrifugation. The preparations were found to consist almost entirely of tubulin and to contain negligibly smallamounts of microtubule-associated proteins. This tubulin polymerizedto make microtubules of ordinary dimensions. The formed microtubules appear to be in labileequilibrium with free tubulin dimer at all temperatures observed. In a buffer consisting of 0.1 M 1, 4-piperazinediethanesulfonic acid, 2 mM dithioerythritol, 1 mM MgS04, 2 mM ethylene glycol bis (/3-aminoethyl ether)-i'V, 7V,,/V/, A7-tetraacetic acid, and 1 mM guanosine5'-triphosphate, pH 6.9, the tubulin of P. borchgrevinki has a critical concentration for assembly of 0.046 (^ 0.008) mg/mL at 35 C and 0.74 (±0.15) mg/mL at the habitat temperature of the fish,-1.8 C. The (deal concentration measured at the lower temperature is quite smallrelative to the critical concentration