Computer-assisted measurement of hyperactivation in human spermatozoa: differences between European and American versions of the Hamilton-Thorn motility analyser.
Computer-assisted measurement of hyperactivation in human spermatozoa: differences between European and American versions of the Hamilton-Thorn motility analyser.
复制标题
计算机辅助测量人类精子过度活跃：欧洲和美国版本的汉密尔顿-索恩活力分析仪之间的差异。
DOI：
10.1093/oxfordjournals.humrep.a138527
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发表时间：
1994
影响因子：
6.1
通讯作者：
I. Cooke
中科院分区：
文献类型：
--
作者：
J. Zhu;A. Pacey;C. Barratt;I. Cooke
使用两种版本的汉密尔顿-索氏运动分析仪（HTMA）以不同的帧率运行，分析来自已证实生育能力的健康供体的精子的过度激活百分比、平均曲线速度（VCL）、平均递进速度（VSL）、侧头位移幅度（ALH）和线性百分比（LIN）。使用30 Hz的机器开发的排序分数标准与使用25 Hz帧率的机器开发的相比，给出了显示运动模式伴随过度激活的精子百分比的可比数字。当使用30赫兹机器制定的标准转移到使用25赫兹机器时，观察到统计学上显著的差异。VSL和VCL的帧率依赖性被认为是造成这些差异的原因；ALH和LIN的值基本上不受帧速率的影响。这些结果对使用欧洲版本的HTMA（25赫兹）的工作人员有启示，这些分类标准包含帧率相关变量，这些变量是由美国同事使用更高帧率的机器开发的。对使用欧洲（25赫兹）机器的工人提出了更合适的分类标准。
Spermatozoa from healthy donors of proven fertility were analysed for percentage hyperactivation, mean curvilinear velocity (VCL), mean progressive velocity (VSL), amplitude of lateral head displacement (ALH) and percentage linearity (LIN) using two versions of the Hamilton-Thorn motility analyser (HTMA) running at different frame rates. The sort fraction criteria developed using a machine utilizing 30 Hz gave comparable figures for the percentage spermatozoa displaying motility patterns concomitant with hyperactivation when compared to that developed for a machine using a frame rate of 25 Hz. Statistically significant differences were observed when criteria developed using 30 Hz machines were transferred to those using 25 Hz. The frame rate dependency of VSL and VCL are thought to account for these differences; values for ALH and LIN were largely unaffected by frame rate. These results have implications for workers using the European version of the HTMA (at 25 Hz) with sort criteria, containing frame rate-dependent variables, which have been developed by American colleagues using the machines of a higher frame rate. More appropriate sort criteria for workers using European (25 Hz) machines are suggested.