Rat myometrial smooth muscle cells express endothelial nitric oxide synthase.
Rat myometrial smooth muscle cells express endothelial nitric oxide synthase.
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大鼠子宫平滑肌细胞表达内皮一氧化氮合酶。
DOI：
10.1093/humrep/12.3.561
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Yallampalli,C
中科院分区：
文献类型：
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作者：
Gangula,PR;Dong,YL;Yallampalli,C
我们检测了培养的大鼠子宫内膜细胞是否产生一氧化氮（NO）并表达各种一氧化氮合成酶（NOS）。将妊娠第18天分离的大鼠子宫内膜细胞与各种NOS刺激剂和抑制剂孵育24和48 h，通过测定培养基中的亚硝酸盐和细胞裂解物中的NOS蛋白来评估NO的产生。N(G)-甲基- l-精氨酸（L-NMMA）抑制子宫内膜细胞产生NO。这种抑制作用被l -精氨酸（3mm）逆转。白细胞介素-1 β (il -1 β)以剂量依赖的方式显著刺激NO的产生。NOS抑制剂L-NMMA可抑制il -1 β刺激的NO生成，而l -精氨酸可逆转其作用。在新分离的子宫肌瘤细胞中检测到丰富的NOS III蛋白，并且在胎牛血清（胎牛血清；10%）存在的培养中保持这种情况。在没有FBS的情况下，NOS III水平在24小时内显著下降（90%）。相比之下，在新鲜分离的肌肉细胞和没有il -1 β培养的细胞中检测不到NOS I和NOS II蛋白。然而，这些细胞中的NOS II蛋白是由il -1 β诱导的。因此，NO是由子宫肌层细胞通过NOS III亚型产生的，子宫肌层NO可能在维持妊娠期间子宫平静中起重要作用。
We examined if rat myometrial cells in culture generate nitric oxide (NO) and express various isoforms of NO synthase (NOS). Myometrial cells isolated from rats on day 18 of gestation were incubated with various stimulators and inhibitors of NOS for 24 and 48 h, and NO production was evaluated by measuring nitrites in the media and NOS proteins in the cell lysates. NO was produced by myometrial cells and its production inhibited by N(G)-methyl-L-arginine (L-NMMA). This inhibition was reversed by L-arginine (3 mM). Interleukin-1beta (IL-1beta) significantly stimulated NO production, in a dose-dependent manner. The IL-1beta-stimulated NO production was inhibited by the NOS inhibitor, L-NMMA, whose effects were reversed by L-arginine. Abundant NOS III protein was detectable in freshly isolated myometrial cells, and this was maintained in culture in the presence of fetal bovine serum (FBS; 10%). In the absence of FBS, NOS III levels decreased significantly (by 90%) within 24 h. In contrast, NOS I and NOS II proteins were undetectable in freshly isolated muscle cells and in cells cultured without IL-1beta. However, NOS II protein in these cells was induced by IL-1beta. Thus, NO is produced by myometrial cells through the NOS III isoform, and the myometrial NO may be important in maintaining uterine quiescence during pregnancy.