Modification of the deoxy-myoglobin/carbonmonoxy-myoglobin UV-vis assay for reliable determination of CO-release rates from organometallic carbonyl complexes
Modification of the deoxy-myoglobin/carbonmonoxy-myoglobin UV-vis assay for reliable determination of CO-release rates from organometallic carbonyl complexes
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DOI：
10.1039/c0dt01809k
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发表时间：
2011-01-01
影响因子：
4
通讯作者：
Fairlamb, Ian J. S.
中科院分区：
文献类型：
--
作者：
Atkin, Anthony J.;Lynam, Jason M.;Fairlamb, Ian J. S.
脱氧肌红蛋白 (deoxy-Mb)/碳单氧肌红蛋白 (Mb-CO) UV-vis 测定是用于量化可能具有治疗效果的 CO 释放分子 (CO-RM) 的 CO 释放速率的主要方法。当使用 Mb-CO 测定法测试具有新颖结构的 CO-RM 时，出现了一些问题，这里首次全面讨论这些问题。本文提出了两种处理测定产生的原始紫外-可见光谱数据的方法。
The deoxy-myoglobin (deoxy-Mb)/carbonmonoxy-myoglobin (Mb-CO) UV-vis assay is the principal method used for quantifying the rates of CO release from CO-releasing molecules (CO-RMs) that might possess therapeutic benefits. Some issues emerge when the Mb-CO assay is utilized for testing CO-RMs with novel structures, which are comprehensively discussed here for the first time. Two methods for processing raw UV-vis spectroscopic data generated from the assay are presented in this paper.