Chapter 4:Non-linear Characterizations of Surface Charge and Interfacial Morphology
Chapter 4:Non-linear Characterizations of Surface Charge and Interfacial Morphology
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第 4 章：表面电荷和界面形貌的非线性表征
DOI：
10.1039/9781849733366-00045
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
S. Stanciu
中科院分区：
文献类型：
--
作者：
S. Lang;G. Stanciu;S. Stanciu
本章讨论了一种测量电改性生物材料表面和亚表面电荷的特殊技术。此外，还介绍了激光扫描显微镜技术在生物材料和生物材料表面和界面表征中的应用。电晕极化/充电是一种在极性材料中引入极化，在非极性材料中引入空间电荷的一种非常有用的方法。所需的仪器相对便宜，并且可以改变大量的参数。激光强度调制法(LIMM)是分析电晕电荷沉积在材料表面附近区域的极化/空间电荷的一种很好的技术。初步实验研究了电晕极化、充电时间、充电温度以及在不同介质中储存对电荷损失的影响。基于二次谐波成像的激光扫描显微镜是研究生物样品表面结构和界面性质的一种非常有用的工具。在共焦显微镜的情况下，紫外光和可见光的强烈散射和吸收导致当组织中的穿透深度大于几十微米时，强度和对比度迅速损失。与共聚焦显微镜不同的是，在SH成像的情况下，扫描光能够穿透到样品体积内更深的地方。
This Chapter discusses a special technique for the measurement of surface and subsurface charge in electrically modified biomaterials. In addition, it also describesthe laser scanning microscopy technique used in surface and interfacial characterisation of biomaterials and biological materials. Corona poling/charging is a very useful method for introducing polarization into polar materials and space charge in non-polar ones. The apparatus required is relatively inexpensive and a large number of parameters can be varied. The laser intensity modulation method (LIMM) is an excellent technique for analysis of the polarization/space charge deposited in regions near the surface of the materials by corona charging. Preliminary experiments were conducted to show the effects of corona polarity, charging time and temperature, and charge loss by storage in different media. Laser scanning microscopy based on second harmonic (SH) imaging represents a very useful tool for investigating the surface structure and the interfacial properties of the biological samples. The combined strong scattering and absorption of ultraviolet and visible light in the case of confocal microscopy lead to a rapid loss of both intensity and contrast when the penetration depth in the tissues is higher than a few tens of microns into tissue. Unlike in confocal microscopy, in the case of the SH imaging the scanning light is able to penetrate much deeper inside the sample's volume.