Circadian regulation of uroguanylin and guanylin in the rat intestine.
Circadian regulation of uroguanylin and guanylin in the rat intestine.
复制标题
大鼠肠道中尿鸟苷蛋白和鸟苷蛋白的昼夜节律调节。
DOI：
10.1152/ajpcell.1999.277.6.c1177
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Jin,WH
中科院分区：
文献类型：
--
作者：
Scheving,LA;Jin,WH
尿鸟苷蛋白 (UGN) 和鸟苷蛋白 (GN) 是鸟苷酸环化酶 C (GC-C) 的内源性肠道配体。我们通过 Northern blot 分析检查了年轻成年大鼠空肠、回肠和近端结肠中 UGN、GN 和 GC-C 的昼夜节律表达。这些测定表明，UGN 在近端小肠中更为丰富，而 GN 和 GC-C 在近端结肠中更为丰富。 mRNA水平显示UGN（3至18倍峰/谷差异）、GN（2.1至2.8倍峰/谷差异）和GC-C（3至5倍峰/谷差异）的显着昼夜节律变化。所有三种 mRNA 的最大丰度都出现在黑暗期，尽管 UGN 和 GN 表达峰值出现在空肠的黑暗期（相对于回肠和结肠）较晚。使用针对 UGN 和 GN 激素原的单特异性多克隆抗体进行的免疫印迹分析证实了 Northern 测定检测到的区域和昼夜节律变化。因此，这些基因的表达不仅受到组织学位置的调节，而且还受到昼夜节律时间的调节。
Uroguanylin (UGN) and guanylin (GN) are the endogenous intestinal ligands for guanylyl cyclase C (GC-C). We examined the circadian expression of UGN, GN, and GC-C in the jejunum, ileum, and proximal colon of young adult rats by Northern blot analyses. These assays revealed that UGN is more abundant in the proximal small intestine, whereas GN and GC-C are more abundant in the proximal colon. mRNA levels showed significant circadian variation for UGN (3- to 18-fold peak/trough difference), GN (2.1- to 2.8-fold peak/trough difference), and GC-C (3- to 5-fold peak/trough difference). The maximal abundance occurred in the dark period for all three mRNAs, although peak UGN and GN expression occurred later in the dark period in the jejunum relative to the ileum and colon. Immunoblot analyses using monospecific polyclonal antibodies against UGN and GN prohormones confirmed the regional and circadian variation detected by Northern assays. Thus the expression of these genes is regulated not only by histological position but also by circadian time.