Behaviour of mesenchymal stem cells, fibroblasts and osteoblasts on smooth surfaces
Behaviour of mesenchymal stem cells, fibroblasts and osteoblasts on smooth surfaces
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DOI：
10.1016/j.actbio.2010.12.033
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发表时间：
2011-04-01
影响因子：
9.7
通讯作者：
Layrolle, Pierre
中科院分区：
文献类型：
--
作者：
Lavenus, Sandrine;Pilet, Paul;Layrolle, Pierre
了解细胞和表面之间的相互作用对于组织工程和生物材料领域至关重要。本研究旨在比较人间充质干细胞 (hMSC)、成骨细胞系 (MC3T3-E1) 和牙龈成纤维细胞 (HGF-1) 在组织培养聚苯乙烯 (TCPS)、玻璃和钛 (Ti) 上的粘附、增殖和分化。 TCPS、玻璃和Ti的平均表面粗糙度分别为5、0.2和40×10(-3)μm。免疫细胞化学和图像分析使得量化贴壁细胞的数量和形态以及焦点的密度成为可能。无论基质如何，hMSC 和成骨细胞均主要呈分支状。 HGF-1 在 Ti 上的焦点数量明显多于在 TCPS 和玻璃上的焦点数量。茜素红定量表明 hMSC 和成骨细胞在 TCPS 上比在 Ti 和玻璃上分化程度更高。基底的表面特性，如粗糙度、润湿性和化学成分，调节细胞的行为。细胞粘附等早期事件可能会影响 hMSC 的分化，从而影响植入生物材料周围的组织愈合。 (C) 2011 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
Understanding of the interactions between cells and surfaces is essential in the field of tissue engineering and biomaterials. This study aimed to compare the adhesion, proliferation and differentiation of human mesenchymal stem cells (hMSCs), an osteoblast cell line (MC3T3-E1) and gingival fibroblasts (HGF-1) on tissue culture polystyrene (TCPS), glass and titanium (Ti). The average surface roughness was 5, 0.2 and 40 x 10(-3) mu m for TCPS, glass and Ti, respectively. Immunocytochemistry and image analysis made it possible to quantify the number and morphology of adherent cells as well as the density of the focal points. Regardless of the substrate, both hMSCs and osteoblastic cells were mainly branch-shaped. HGF-1 exhibited a significantly higher number of focal points on Ti than on TCPS and glass. Alizarin red quantification indicated that both hMSCs and osteoblastic cells were more differentiated on TCPS than on Ti and glass. The surface properties of substrates, such as roughness, wettability and chemical composition, modulated the behaviour of the cells. Early events, such as cell adhesion, may influence the differentiation of hMSC and consequently tissue healing around implanted biomaterials. (C) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.