An efficient environmental DNA detection method for rare species: a case study of a small salamander (Hynobius boulengeri)
An efficient environmental DNA detection method for rare species: a case study of a small salamander (Hynobius boulengeri)
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一种高效的稀有物种环境DNA检测方法：以小蝾螈（Hynobius bouulengeri）为例
DOI：
10.1007/s44211-023-00289-6
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发表时间：
2023
影响因子：
1.6
通讯作者：
Minamoto Toshifumi
中科院分区：
文献类型：
--
作者：
Sakata Masayuki K.;Takeshita Daiki;Nishizawa Ryohei;Sato Takuya;Minamoto Toshifumi
生物多样性的丧失是一个严重的问题，两栖动物尤其受到威胁。日本的大部分小蝾螈都濒临灭绝。分布信息是保护这些稀有物种的基础;然而，小蝾螈通常很难找到或捕捉。环境DNA分析是监测这类珍稀物种的一种有效的调查方法。常规的聚合酶链反应（PCR）方法（其将PCR扩增与随后的电泳相结合）和实时PCR方法（其使用荧光材料）通常用于此目的。在这项研究中，这两种检测方法进行了比较，使用一种罕见的蝾螈物种，小鲵boulengeri，作为模式案例。我们比较了三点：（i）检测灵敏度，（ii）与检测有关的环境因素的影响，以及（iii）两种方法的时间和财务成本。为了进行这种比较，我们开发了一种实时PCR检测方法，进行了实地调查，并比较了常规和实时PCR方法的时间和财务成本。结果表明，两种方法的检测灵敏度差异不显著，环境因素的影响也趋于一致。此外，常规PCR方法的财务成本较低，而实时PCR方法的时间成本较低。因此，根据目的、时间和经济成本选择eDNA检测方法将促进有效的监测，并有助于保护稀有物种。
Loss of biodiversity is a serious concern, and amphibians are particularly threatened. Most small salamanders in Japan are endangered. Distributional information is fundamental to the conservation of these rare species; however, small salamanders are generally difficult to locate or catch. Environmental DNA analysis is an effective survey method for monitoring such rare species. The conventional polymerase chain reaction (PCR) method, which combines PCR amplification with subsequent electrophoresis, and the real-time PCR method, which uses fluorescent material, are commonly used for this purpose. In this study, a comparison of these two detection methods was conducted using a rare salamander species,Hynobius boulengeri, as a model case. We compared three points: (i) detection sensitivity, (ii) influence of environmental factors related to detection, and (iii) time and financial costs of the two methods. To perform this comparison, we developed a real-time PCR detection assay, conducted field surveys, and compared the time and financial costs of conventional and real-time PCR methods. The comparison showed no statistical difference in the detection sensitivity from field samples, and the effects of environmental factors tended to be similar. In addition, the financial cost was lower for the conventional PCR method while the time cost was lower for the real-time PCR method. Therefore, selecting eDNA detection methods based on objectives, time, and financial costs will promote efficient monitoring and contribute to the conservation of rare species.Graphical Abstract
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影响因子：
4.6
作者：
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通讯作者：
Buchmann K
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影响因子：
2.7
作者：
Takeshita Daiki;Terui Shigeharu;Ikeda Kousuke;Mitsuzuka Takashi;Osathanunkul Maslin;Minamoto Toshifumi
通讯作者：
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DOI：
10.3354/esr01073
发表时间：
2020
期刊：
Endang Species Res
影响因子：
--
作者：
Toshiaki Jo;Sei Tomita;Yukihiro Kohmatsu;Maslin Osathanunkul;Atushi Ushimaru;Toshifumi Minamoto
通讯作者：
Toshifumi Minamoto