Expression of Sphingosine Kinase-1 Is Associated with Invasiveness and Poor Prognosis of Oral Squamous Cell Carcinoma
Expression of Sphingosine Kinase-1 Is Associated with Invasiveness and Poor Prognosis of Oral Squamous Cell Carcinoma
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DOI：
10.21873/anticanres.12359
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发表时间：
2018-03-01
影响因子：
2
通讯作者：
Ueda, Yoshimichi
中科院分区：
文献类型：
--
作者：
Kato, Koichiro;Shimasaki, Miyako;Ueda, Yoshimichi
背景/目的：鞘氨醇激酶-1(SphK1)在多种肿瘤中的表达已有报道。然而，SphK1在癌症进展中的确切作用仍不清楚。本研究旨在研究SphK1在口腔鳞状细胞癌(OSCC)中的表达，探讨SphK1在口腔鳞状细胞癌(OSCC)增殖和侵袭中的作用及其预后意义。材料和方法：采用免疫组织化学和免疫印迹法检测69例口腔鳞癌组织中SphK1、E-钙粘素、波形蛋白和Ki-67的表达，分析其与口腔鳞癌侵袭性和预后的关系。结果：69例口腔鳞状细胞癌中有38例表达SphK1，尤其是在侵袭前沿。SphK1高表达的口腔鳞癌患者具有较高的侵袭性分级和不良生存率。口腔鳞癌组织中SphK1的表达与波形蛋白表达的获得和E-钙粘蛋白表达的缺失有关，其Ki-67标记指数在高表达和低表达的口腔鳞癌中差异无统计学意义。结论：SphK1参与口腔鳞状细胞癌的侵袭和不良预后，提示其在口腔鳞癌细胞上皮间充质转化过程中起重要作用。
Background/Aim: The expression of sphingosine kinase-1 (SphK1) has been reported in several cancers. However, the exact roles of SphK1 in cancer progression still remain unknown. The aim of the present study was to investigate SphK1 expression in oral squamous cell carcinoma (OSCC) and clarify the involvement of SphK1 in the proliferation and invasiveness of OSCC and its prognostic implications. Materials and Methods: Expression of SphK1, E-cadherin, vimentin, and Ki-67 were examined in 69 OSCC tissues immunohistochemically, as well as by western blot, and correlations between their expression and relationships with tumor invasiveness and prognosis were analyzed. Results: SphK1 was expressed in the tumor cells of 38 of 69 OSCCs, particularly at the invasion front. Patients with OSCCs with high SphK1 expression showed higher invasive grades and unfavorable survival rates. SphK1 expression correlated with acquisition of vimentin expression and loss of E-cadherin expression; there was no significant difference in Ki-67 labeling indices between OSCCs with high and low SphK1 expression. Conclusion: These results demonstrate the involvement of SphK1 in the invasiveness of OSCC and in unfavorable prognosis, indicating its role in the epithelial-mesenchymal transition of OSCC cells.