Involvement of pyruvate oxidase activity and acetate production in the survival of Lactobacillus plantarum during the stationary phase of aerobic growth
Involvement of pyruvate oxidase activity and acetate production in the survival of Lactobacillus plantarum during the stationary phase of aerobic growth
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DOI：
10.1128/aem.00659-06
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发表时间：
2006-12-01
影响因子：
4.4
通讯作者：
Hols, Pascal
中科院分区：
文献类型：
--
作者：
Goffin, Philippe;Muscariello, Lidia;Hols, Pascal
除了先前表征的丙酮酸氧化酶PoxB之外，植物乳杆菌基因组编码四种预测的丙酮酸氧化酶（PoxC、PoxD、PoxE和PoxF）。每个丙酮酸氧化酶基因单独失活，只有poxF的敲除导致丙酮酸氧化酶活性在测试条件下降低。我们在这里表明，L。plantarum有两种主要的丙酮酸氧化酶：PoxB和PoxF。两者都参与乳酸转化为乙酸在早期稳定阶段的有氧生长，并调节碳代谢产物的镇压。通过敲除poxB和poxF基因构建了缺乏丙酮酸氧化酶活性的菌株。在该突变体中，乙酸盐的产生受到强烈影响，乳酸盐仍然是葡萄糖或麦芽糖发酵的主要终产物。值得注意的是，稳定期期间的存活率似乎在poxB poxF双突变体中显著提高。
In addition to the previously characterized pyruvate oxidase PoxB, the Lactobacillus plantarum genome encodes four predicted pyruvate oxidases (PoxC, PoxD, PoxE, and PoxF). Each pyruvate oxidase gene was individually inactivated, and only the knockout of poxF resulted in a decrease in pyruvate oxidase activity under the tested conditions. We show here that L. plantarum has two major pyruvate oxidases: PoxB and PoxF. Both are involved in lactate-to-acetate conversion in the early stationary phase of aerobic growth and are regulated by carbon catabolite repression. A strain devoid of pyruvate oxidase activity was constructed by knocking out the poxB and poxF genes. In this mutant, acetate production was strongly affected, with lactate remaining the major end product of either glucose or maltose fermentation. Notably, survival during the stationary phase appeared to be dramatically improved in the poxB poxF double mutant.