Complete covalent structure of nisin Q, new natural nisin variant, containing post-translationally modified amino acids
Complete covalent structure of nisin Q, new natural nisin variant, containing post-translationally modified amino acids
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DOI：
10.1271/bbb.80066
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发表时间：
2008-07-01
影响因子：
1.6
通讯作者：
Sonomoto, Kenji
中科院分区：
文献类型：
--
作者：
Fukao, Masanori;Obita, Takayuki;Sonomoto, Kenji
乳酸乳球菌61-14产生的nisin变体的第三个成员nisin Q是一种核糖体合成的抗菌肽，即所谓的lantibiotic，含有翻译后修饰的氨基酸，如衣硫氨酸和脱氢丙氨酸。本文采用二维H-1核磁共振（NMR）技术确定了nisin Q由34个氨基酸组成的完整共价结构。利用全相关光谱（TOCSY）和核Overhauser增强光谱（NOESY）对含有异常氨基酸的nisin Q进行序列鉴定。nisin Q中观察到的远程核Overhauser效应（NOE）表明分子内桥接残基3-7、8-11、13-19、23-26和25-28的所有五组硫氨酸都被分配。因此，与nisin A相比，nisin Q的共价结构与其他两种nisin具有相同的硫醚键形成，但包含四个氨基酸取代。
The third member of the nisin variant, nisin Q, produced by Lactococcus lactis 61-14, is a ribosomally-synthesized antimicrobial peptide, the so-called lantibiotic containing post-translationally modified amino acids such as lanthionine and dehydroalanine. Here, we determined the complete covalent structure of nisin Q, consisting of 34 amino acids, by two-dimensional H-1 nuclear magnetic resonance (NMR) spectroscopy. Sequential assignment of nisin Q containing the unusual amino acids was performed by total correlation spectroscopy (TOCSY) and nuclear Overhauser enhancement spectroscopy (NOESY). The observed long range nuclear Overhauser effect (NOE) in nisin Q indicated assignment of all five sets of lanthionines that intramolecularly bridge residues 3-7, 8-11, 13-19, 23-26, and 25-28. Consequently, the covalent structure of nisin Q was determined to hold the same thioether linkage formation as the other two nisins, but to harbor the four amino acid substitutions, in contrast with nisin A.