Limiting photoreceptor death and deconstruction during experimental retinal detachment: The value of oxygen supplementation
Limiting photoreceptor death and deconstruction during experimental retinal detachment: The value of oxygen supplementation
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DOI：
10.1016/s0002-9394(99)00104-x
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发表时间：
1999-08-01
影响因子：
4.2
通讯作者：
Stone, J
中科院分区：
文献类型：
--
作者：
Mervin, K;Valter, K;Stone, J
目的：评估缺氧在导致脱落视网膜中光感受器死亡和解构的作用以及补充氧在限制这种损害中的有效性。方法：视网膜脱离在10只猫的右眼中通过手术诱导了视网膜脱离。允许猫在手术中存活3天。在这3天中，有两个在常氧（房间空气，21％的氧气）中保存，而高氧（70％氧）保存。通过使用标签用于正常和碎片的DNA，用抗体和锥形鞘特异性凝集素检查视网膜死亡，以证明其内部和外部段的状态，外丛状层的突触结构，以及的分布基本的成纤维细胞生长因子（BFGF）和原位杂交以证明BFGF mRNA。退回：视网膜脱离没有补充氧导致某些光感受器的死亡；从内部段中的细胞色素氧化酶的丧失和幸存感光体的外部段的塌陷；突触神经素轮廓的丧失来自外丛状层；以及视网膜神经元和神经元中BFGF蛋白的丧失，而不是视网膜血管。脱离期间补充氧气（高氧）减轻了所有这些变化，减少了光感受器的死亡，维持幸存的光感受器的专业结构，并稳定视网膜内的BFGF。养分的来源是诱导感光体死亡和解构的因素以及在脱离视网膜的BFGF丧失时，在视网膜脱离和手术诊断之间向人类患者提供的高氧可以增强重新连接的视网膜的功能。 （c）1999年，Elsevier Science Inc.保留所有权利。
PURPOSE: To assess the role of hypoxia in causing the death and deconstruction of photoreceptors in detached retinas and the effectiveness of supplemental oxygen in limiting such damage.METHODS: Retinal detachment was induced surgically in the right eye of each of 10 cats. The cats were allowed to survive surgery for 3 days. Two were kept for these 3 days in normoxia (room air, 21% oxygen) and eight in hyperoxia (70% oxygen). The retinas were examined for cell death by use of labels for normal and fragmenting DNA, with antibodies and a cone sheath-specific lectin to demonstrate the status of their inner and outer segments, the synaptic structures of the outer plexiform layer, and the distribution of basic fibroblast growth factor (bFGF) and with in situ hybridization to demonstrate bFGF mRNA.RESULTS: Retinal detachment without oxygen supplementation caused the death of some photoreceptors; the loss of cytochrome oxidase from the inner segments and the collapse of the outer segments of surviving photoreceptors; the loss of synaptophysin profiles from the outer plexiform layer; and the loss of bFGF protein from retinal neurons and neuroglia but not from retinal vessels. Oxygen supplementation (hyperoxia) during detachment mitigated all these changes, reducing photoreceptor death, maintaining the specialized structures of surviving photoreceptors, and stabilizing the bFGF within the retina.CONCLUSIONS: In experimental retinal detachment, hypoxia caused by the separation of outer retina from its normal source of nutrients is a factor in inducing the death and deconstruction of photoreceptors as well as in the loss of bFGF from the detached retina, Hyperoxia offered to human patients between diagnosis of retinal detachment and surgery may enhance the function of the reattached retina. (C) 1999 by Elsevier Science Inc. All rights reserved.