Interleukin-8, monocyte chemoattractant protein-1 and IL-10 in the vitreous fluid of patients with proliferative diabetic retinopathy
Interleukin-8, monocyte chemoattractant protein-1 and IL-10 in the vitreous fluid of patients with proliferative diabetic retinopathy
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DOI：
10.1111/j.1464-5491.2005.01538.x
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发表时间：
2005-06-01
影响因子：
3.5
通讯作者：
Simó, R
中科院分区：
文献类型：
--
作者：
Hernández, C;Segura, RM;Simó, R
目的 测定增殖性糖尿病视网膜病变 (PDR) 患者玻璃体内两种促炎细胞因子 [白细胞介素 8 (IL-8)、单核细胞趋化蛋白 1 (MCP-1)] 和抗炎细胞因子白细胞介素 10 (IL-10) 的玻璃体内水平。此外，还评估了细胞因子谱与 PDR 活性之间的关系。 患者和方法 该研究包括 22 名连续接受玻璃体切除术的 PDR 糖尿病患者（4 名 1 型和 18 名 2 型）。十六名年龄匹配的非糖尿病患者，患有其他需要玻璃体切除术的疾病，但视网膜未直接受到新生血管的影响，作为对照组。通过酶联免疫吸附测定（ELISA）测定IL-8、MCP-1和IL-10。结果与对照组相比，患有PDR的糖尿病患者玻璃体中IL-8和MCP-1的水平显着升高[173.5（64-1670）vs. 49 pg/ml（25-145），P < 0.001和2171 (388-6155) vs. 438 pg/ml (207-1344)，P < 0.001，分别]。此外，活动性 PDR 患者的玻璃体 IL-8 和 MCP-1 浓度高于静态 PDR 患者 [324.5 (80-1670) vs. 173.5 pg/ml (64-487)，P = 0.06 和 3596 (16706155) vs. 1143 pg/ml (388-2500)， P = 0.01，分别]。然而，糖尿病患者玻璃体中IL-10的水平与对照组相似[2.89（1.55-5.50）与2.46 pg/ml（2.2-5.41），P = NS]。 结论 PDR患者玻璃体液中促炎细胞因子IL-8和MCP-1增加，但抗炎细胞因子IL-10增加。此外，玻璃体内IL-8和MCP-1水平与PDR活性相关，因此表明这些细胞因子在PDR中可能具有重要的致病作用。
Aims To determine the intra-vitreous levels of two pro-inflammatory cytokines [interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1)] and the anti-inflammatory cytokine interleukin-10 (IL-10) in patients with proliferative diabetic retinopathy (PDR). In addition, the relationship between the profile of cytokines and PDR activity has also been evaluated.Patients and methods The study included 22 consecutive diabetic patients with PDR (4 Type 1 and 18 Type 2) on whom a vitrectomy was performed. Sixteen age-matched non-diabetic patients with other conditions requiring vitrectomy, but in which the retina was not directly affected by neovascularization served as a control group. IL-8, MCP-1 and IL-10 were measured by enzyme-linked immunosorbent assay (ELISA).Results The vitreal levels of both IL-8 and MCP-1 were strikingly higher in diabetic patients with PDR in comparison with the control group [173.5 (64-1670) vs. 49 pg/ml (25-145), P < 0.001, and 2171 (388-6155) vs. 438 pg/ml (207-1344), P < 0.001, respectively]. In addition, the vitreous concentrations of IL-8 and MCP-1 were higher in patients with active PDR than in those patients with quiescent PDR [324.5 (80-1670) vs. 173.5 pg/ml (64-487), P = 0.06 and 3596 (16706155) vs. 1143 pg/ml (388-2500), P = 0.01, respectively]. However, vitreal levels of IL-10 in diabetic patients were similar to that obtained in the control group [2.89 (1.55-5.50) vs. 2.46 pg/ml (2.2-5.41), P = NS].Conclusions The pro-inflammatory cytokines IL-8 and MCP-1 are increased in the vitreous fluid of PDR patients without an increase in the anti-inflammatory cytokine IL-10. In addition, both IL-8 and MCP-1 intra-vitreous levels correlated with PDR activity, thus suggesting that these cytokines may be pathogenically important in PDR.