Characterization of three isotypes of immunoglobulin light chains and T-cell antigen receptor α in zebrafish
Characterization of three isotypes of immunoglobulin light chains and T-cell antigen receptor α in zebrafish
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DOI：
10.1007/s002510000229
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发表时间：
2000-09-01
期刊：
影响因子：
3.2
通讯作者：
Litman, GW
中科院分区：
文献类型：
--
作者：
Haire, RN;Rast, JP;Litman, GW
斑马鱼（Danio rerio）已成为了解基因表达的发育调控的重要模型，并且在表征免疫系统的发育方面具有相当大的潜力。使用许多不同的方法，包括异源杂交和短引物PCR，三种不同类型的轻链基因的cDNA进行了鉴定和表征。斑马鱼轻链分别与鳟鱼1型、鳟鱼2型和鲶鱼F型相似。还鉴定和表征了T细胞抗原受体α（TCR α）。在免疫球蛋白和TCR cDNA检测中，发现了大量的异常转录本，包括不育转录本、生殖系VJC转录本、异常剪接形式和V-V转录本。轻链和TCR α TCR基因座各自由V基因区段的多个家族组成，甚至从测序的每种同种型的少量cDNA中也显而易见。报告的基因序列提供了一套必要的标记B-和T-细胞谱系，这将有助于调查免疫系统的发展。
The zebrafish (Danio rerio) has become a significant model for understanding the developmental regulation of gene expression and holds considerable potential for characterizing the development of the immune system. Using a number of different approaches, including heterologous hybridization and short-primer PCR, cDNAs for three different classes of light-chain genes were identified and characterized. The zebrafish light chains are similar to trout type 1, trout type 2, and catfish type F, respectively. T-cell antigen receptor alpha (TCR alpha) was also identified and characterized. A high proportion of unusual transcripts including sterile transcripts, germline VJC transcripts, aberrant splice forms, and V-V transcripts were encountered ill the immunoglobulin and TCR cDNAs examined. The light-chain and TCRa TCR loci each consist of multiple families of V gene segments, apparent even from the small numbers of cDNAs of each isotype sequenced. The gene sequences reported provide an essential set of markers of both B- and T-cell lineages that will facilitate investigations of immune system development.