The fine specificity of monoclonal anti-DNA antibodies induced in normal mice by immunization with bacterial DNA.
The fine specificity of monoclonal anti-DNA antibodies induced in normal mice by immunization with bacterial DNA.
复制标题
通过细菌 DNA 免疫在正常小鼠中诱导单克隆抗 DNA 抗体的良好特异性。
DOI：
10.1006/jaut.1993.1002
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发表时间：
1993
影响因子：
12.8
通讯作者：
Gilkeson,GS
中科院分区：
文献类型：
--
作者：
Pyun,EH;Pisetsky,DS;Gilkeson,GS
为了进一步评价细菌DNA免疫作为研究抗DNA应答中抗原驱动的模型，表征了诱导的单克隆抗DNA抗体的特异性。用弗罗姆单链DNA免疫BALB/c小鼠，获得一组IgM和IgG单克隆抗DNA抗体。在完全弗氏佐剂中与甲基化牛血清白蛋白复合。通过ELISA测试这些抗体与DNA和非DNA抗原的结合，以评估其多特异性范围。发现这些单克隆抗体与核酸以及非核酸抗原如β-半乳糖苷酶、心磷脂、Ro、La和Sm结合。这些研究表明，来自正常小鼠的抗DNA抗体，虽然由细菌DNA诱导，但可能显示出广泛的抗原识别，因此类似于狼疮抗DNA抗体，其中许多是多特异性的，在其交叉反应性模式中。
To evaluate further bacterial DNA immunization as a model to study antigen drive in the anti-DNA response, the specificity of induced monoclonal anti-DNA antibodies was characterized. A panel of IgM and IgG monoclonal anti-DNA antibodies was produced from spleen cells of BALB/c mice immunized with single-stranded DNA fromE. colicomplexed to methylated bovine serum albumin in complete Freund's adjuvant. The binding of these antibodies to DNA and non-DNA antigens was tested by ELISA to assess their range of polyspecificity. These monoclonal antibodies were found to bind to nucleic acid as well as non-nucleic acid antigens, such as β-galactosidase, cardiolipin, Ro, La and Sm. These studies demonstrate that anti-DNA antibodies from normal mice, although induced by bacterial DNA, may display a broad range of antigen recognition and thus resemble lupus anti-DNA antibodies, many of which are polyspecific, in their pattern of cross-reactivity.