Quantitative and qualitative analyses of proteoglycans in cartilage extracts by precipitation with 1,9-dimethylmethylene blue
Quantitative and qualitative analyses of proteoglycans in cartilage extracts by precipitation with 1,9-dimethylmethylene blue
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DOI：
10.3109/03008209609028881
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发表时间：
1996-01-01
影响因子：
2.9
通讯作者：
Hanschke, M
中科院分区：
文献类型：
--
作者：
Muller, G;Hanschke, M
染料1，9-二甲基亚甲基蓝可用于从关节软骨提取物中特异性和定量沉淀硫酸化糖胺聚糖以及蛋白聚糖。染料DMMB的消耗使得能够通过测量在595 nm处的吸收的减少来确定提取物中存在的蛋白聚糖的量。DMMB和蛋白聚糖的沉淀复合物用于定性研究琼脂糖平板凝胶中不同蛋白聚糖的电泳迁移率及其印迹后的免疫学表征。由于其更高的灵敏度和更大的简单性，本报告中描述的方法是一个有前途的替代程序的基础上阿辛蓝。（十五）
The dye 1,9-dimethylmethylene blue may be used for the specific and quantitative precipitation of sulphated glycosaminoglycans as well as proteoglycans from the extracts of articular cartilage. The consumption of the dye DMMB enables to determine the amount of the proteoglycans present in the extract, by measuring the decrease of the absorption at 595 nm. The precipitated complex of DMMB and proteoglycans is used for qualitative investigations into the electrophoretic mobility of the different proteoglycans in agarose slab gel and their immunological characterization after blotting. Because of its higher sensitivity and greater simplicity the method described in this report is a promising alternative to the procedure based on alcian blue.(15)