The role of acyl-CoA thioesterase ACOT8I in mediating intracellular lipid metabolism in oleaginous fungus Mortierella alpina
The role of acyl-CoA thioesterase ACOT8I in mediating intracellular lipid metabolism in oleaginous fungus Mortierella alpina
复制标题
酰基辅酶A硫酯酶ACOT8I介导产油真菌高山被孢霉细胞内脂质代谢的作用
DOI：
10.1007/s10295-018-2006-8
复制
发表时间：
2018-04-01
影响因子：
3.4
通讯作者：
Chen, Yong Q.
中科院分区：
文献类型：
--
作者：
Guo, Jing;Chen, Haiqin;Chen, Yong Q.
硫酯酶（TE）在脂肪酸（FA）的代谢中发挥着重要作用。为了探索 TE 在产油真菌高山被孢霉介导细胞内脂质代谢中的作用，酰基辅酶 A 硫酯酶 ACOT8I 被过表达。重组菌株中的总脂肪酸 (TFA) 含量与野生型 M. alpina 相同，而游离脂肪酸 (FFA) 的产量从约 0.9%（野生型）提高到 2.8%（重组型），大约增加了三倍。在重组菌株中，FFA 形式的亚油酸含量占 FFA 级分中 TFA 的约 9%，但在野生型 M. alpina 中仅占约 1.3%。重组菌株中FFA形式的γ-亚麻酸和花生四烯酸含量分别占FFA组分中TFA的约4%和25%，而在野生型高山被孢霉中均未检测到FFA形式的γ-亚麻酸和花生四烯酸含量。 TE ACOT8I 在产油真菌 M. alpina 中的过表达增强了从酰基辅酶 A 到 FFA 的通量，提高了 FFA 的产量并定制了脂质种类的 FA 谱。
Thioesterases (TEs) play an essential role in the metabolism of fatty acids (FAs). To explore the role of TEs in mediating intracellular lipid metabolism in the oleaginous fungus Mortierella alpina, the acyl-CoA thioesterase ACOT8I was overexpressed. The contents of total fatty acids (TFAs) were the same in the recombinant strains as in the wild-type M. alpina, whilst the production of free fatty acids (FFAs) was enhanced from about 0.9% (wild-type) to 2.8% (recombinant), a roughly threefold increase. Linoleic acid content in FFA form constituted about 9% of the TFAs in the FFA fraction in the recombinant strains but only about 1.3% in the wild-type M. alpina. The gamma-linolenic acid and arachidonic acid contents in FFA form accounted for about 4 and 25%, respectively, of the TFAs in the FFA fraction in the recombinant strains, whilst neither of them in FFA form were detected in the wild-type M. alpina. Overexpression of the TE ACOT8I in the oleaginous fungus M. alpina reinforced the flux from acyl-CoAs to FFAs, improved the production of FFAs and tailored the FA profiles of the lipid species.