Cytogenetics of childhood T-cell leukemia
Cytogenetics of childhood T-cell leukemia
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儿童 T 细胞白血病的细胞遗传学
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
DorothyL. Williams
中科院分区：
文献类型：
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作者：
S. Raimondi;F. Behm;P. Roberson;C. Pui;G. Rivera;S. Murphy;DorothyL. Williams
对57例儿童T细胞急性淋巴细胞白血病（ALL）进行了核型分析，以建立ALL的细胞遗传学特征。有三个问题特别令人感兴趣。T细胞ALL的染色体变化是否优先影响编码T细胞抗原受体（TCR）的基因已被定位的条带？B祖细胞ALL中TCR基因区的改变是否以任何显著的频率出现？这种疾病的染色体异常与T细胞个体发育阶段有关吗？一个相对较高比例的情况下（65%）有一个假二倍体核型介绍，大多数（58%）的特点是易位。易位的总频率为44%，与我们实验室观察到的所有带状ALL病例相当。亚二倍体和超二倍体非常罕见（57例中只有4例）; 16例（28%）核型明显正常。在一半的易位病例中（24例中的14例），断点位于α和β链TCR基因已定位的区域。在TCR基因位点附近一致观察到的染色体断裂点为7 q32-q36（TCR β链; n = 8）、14 q11-q13（TCR α链; n = 6）;其他常见断裂点为9 p13-pter（n = 8）和6 q15-qter（n = 9）。与335例B细胞前体ALL患者相比，T细胞病例中TCR基因位点附近的染色体改变发生率更高（26% v1.5%，P = 0.0001）。23例观察到I期胸腺细胞发育（CD 7+、CD 2+、CD 5+、CD 1-、CD 3-、CD 4-、CD 8-），25例观察到II期胸腺细胞发育（CD 7+、CD 2+、CD 5+、CD 1+、CD 3-、CD 4 +/-、CD 8 +/-），9例观察到III期胸腺细胞发育（CD 9+、CD 2+、CD 1-、CD 5+、CD 3+以及CD 4+或CD 8+）。细胞遗传学结果和T细胞个体发育之间的唯一统计学显著关联是I期胸腺细胞病例中正常核型的频率较高，II期病例中假二倍体的频率较高。特定易位与胸腺细胞成熟水平之间无明显关系。我们的研究结果表明，大多数T细胞ALL患儿具有假二倍体核型，尽管令人惊讶的是高比例缺乏可证实的异常克隆。特定的染色体变化似乎与本研究中定义的T细胞个体发育的离散阶段无关，但它们优先发生在含有TCR基因的条带中。
The karyotypes of 57 cases of childhood T-cell acute lymphoblastic leukemia (ALL) were analyzed to establish the cytogenetic profile in this disease. Three questions were of particular interest. Do the chromosomal changes in T-cell ALL preferentially affect bands where genes encoding the T-cell receptor for antigen (TCR) have been mapped? Do alterations involving the TCR gene regions appear with any notable frequency in B-progenitor ALL? Do chromosomal abnormalities in this disease relate to stage of T-cell ontogeny? A relatively high proportion of cases (65%) had a pseudodiploid karyotype at presentation, the majority (58%) characterized by a translocation. The overall frequency of translocations was 44%, comparable to that among all banded cases of ALL seen in our laboratory. Hypodiploidy and hyperdiploidy were exceedingly rare (only four of 57 cases); 16 cases (28%) had apparently normal karyotypes. In half the cases with a translocation (14 of 24), the breakpoints were in regions to which the alpha and beta chain TCR genes have been mapped. Chromosomal breakpoints that were consistently observed in the vicinity of TCR gene loci were 7q32-q36 (TCR beta chain; n = 8), 14q11-q13 (TCR alpha chain; n = 6); other frequent breakpoints were 9p13-pter (n = 8) and 6q15-qter (n = 9). Chromosomal alterations occurred near TCR gene loci significantly more often in T-cell cases than in a comparison group of 335 patients with B-cell precursor ALL (26% v 1.5%, P = .0001). Stage I thymocyte development (CD7+, CD2+, CD5+, CD1-, CD3-, CD4-, CD8-) was noted in 23 cases, stage II (CD7+, CD2+, CD5+, CD1+, CD3-, CD4 +/-, CD8 +/-) in 25 cases, and stage III (CD9+, CD2+, CD1-, CD5+, CD3+, and either CD4+ or CD8+) in nine cases. The only statistically significant associations between cytogenetic findings and T-cell ontogeny were a higher frequency of normal karyotypes in cases with stage I thymocytes, and of pseudodiploidy in stage II cases. There was no apparent relationship between particular translocations and level of thymocyte maturation. Our findings indicate that most children with T-cell ALL have pseudodiploid karyotypes, although a surprisingly high percentage lack demonstrable abnormal clones. Specific chromosomal changes do not appear to be related to discrete stages of T-cell ontogeny as defined in this study, but they occur preferentially in bands containing TCR genes.
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儿童急性淋巴细胞白血病核型与免疫表型的相关性。
DOI：
10.1200/jco.1988.6.1.56
发表时间：
1988
期刊：
Journal of clinical oncology : official journal of the American Society of Clinical Oncology
影响因子：
--
作者：
Pui,CH;Williams,DL;Roberson,PK;Raimondi,SC;Behm,FG;Lewis,SH;Rivera,GK;Kalwinsky,DK;Abromowitch,M;Crist,WM
通讯作者：
Crist,WM
对来自人 T 细胞白血病的含 t(7;9) 细胞系 (SUP-T3) 中的 TCRB 和 ABL 进行分子分析。
DOI：
10.1073/pnas.84.1.251
发表时间：
1987
影响因子：
11.1
作者：
Westbrook,CA;Rubin,CM;LeBeau,MM;Kaminer,LS;Smith,SD;Rowley,JD;Diaz,MO
通讯作者：
Diaz,MO
7 号染色体带 q34 重排 T 细胞肿瘤的临床和生物学特征。
影响因子：
20.3
作者：
Smith,SD;Morgan,R;Gemmell,R;Amylon,MD;Link,MP;Linker,C;Hecht,BK;Warnke,R;Glader,BE;Hecht,F
通讯作者：
Hecht,F
替尼泊苷加阿糖胞苷可改善白细胞计数大于或等于 100 x 10(9)/L 的儿童急性淋巴细胞白血病的预后。
DOI：
10.1200/jco.1987.5.7.1015
发表时间：
1987
期刊：
Journal of clinical oncology : official journal of the American Society of Clinical Oncology
影响因子：
--
作者：
Dahl,GV;Rivera,GK;Look,AT;Hustu,HO;Kalwinsky,DK;Abromowitch,M;Mirro,J;Ochs,J;Murphy,SB;Dodge,RK
通讯作者：
Dodge,RK
大剂量甲氨蝶呤治疗儿童急性淋巴细胞白血病的疗效：当代风险分类分析。
影响因子：
20.3
作者：
Abromowitch,M;Ochs,J;Pui,CH;Fairclough,D;Murphy,SB;Rivera,GK
通讯作者：
Rivera,GK