Procedures for mucosal immunization and analyses of cellular immune response to candidate HIV vaccines in murine and nonhuman primate models.
Procedures for mucosal immunization and analyses of cellular immune response to candidate HIV vaccines in murine and nonhuman primate models.
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DOI：
10.1007/978-1-4939-1115-8_24
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发表时间：
2014-01-01
期刊：
影响因子：
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通讯作者：
Sastry, K Jagannadha
中科院分区：
文献类型：
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作者：
Singh, Shailbala;Nehete, Pramod;Sastry, K Jagannadha
粘膜组织取样和免疫反应分析是针对人类免疫缺陷病毒（HIV）的疫苗开发策略的组成部分，HIV主要通过口腔-生殖器粘膜传播。虽然免疫测定开发和标准化尝试采用小鼠模型，但可外推至人类的免疫原性和保护效力仅从非人灵长类动物的实验中实现。在这里，我们描述了常用的做法，免疫接种恒河猴（猕猴）沿着的程序，获得重要的粘膜组织样本的猕猴和小鼠。我们还描述了适用于小鼠和灵长类动物实验的两种重要测定的详细方案，用于确定疫苗接种后诱导的抗原特异性T细胞反应。
Sampling the mucosal tissues and analyses of immune responses are integral to vaccine-development strategies against human immunodeficiency virus (HIV), which is transmitted predominantly across the oro-genital mucosa. While immune assay development and standardization attempts employ mouse models, immunogenicity and protective efficacy that can be extrapolated to humans are realized only from experiments in nonhuman primates. Here, we describe commonly used practices for immunizations in rhesus macaques (Macaca mulatta) along with procedures for obtaining important mucosal tissues samples from macaques and mice. We also describe detailed protocols for two important assays applicable in mouse as well as primate experiments for determining antigen-specific T cells responses induced after vaccination.