The Nutrient and Energy Pathway Requirements for Surface Motility of Nonpathogenic and Uropathogenic Escherichia coli.
The Nutrient and Energy Pathway Requirements for Surface Motility of Nonpathogenic and Uropathogenic Escherichia coli.
复制标题
非致病性和尿路致病性大肠杆菌表面运动的营养和能量途径要求。
DOI：
10.1128/jb.00467-20
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发表时间：
2021
影响因子：
3.2
通讯作者：
Reitzer,Larry
中科院分区：
文献类型：
--
作者：
Sudarshan,Sushmita;Hogins,Jacob;Ambagaspitiye,Sankalya;Zimmern,Philippe;Reitzer,Larry
尿路致病性大肠杆菌（UPEC）是大多数非复杂性尿路感染（UTIs）的病原体。运动可能是导致这些感染的原因之一，E.大肠杆菌具有鞭毛依赖的游泳运动性、鞭毛依赖的表面运动性（通常称为群集）和最近观察到的菌毛依赖的表面运动性。表面能动性还没有被广泛研究，但对于已被测试的菌株，非致病性E。大肠杆菌（NPEC）实验室菌株使用皮利，这些实验室菌株的NPEC高运动衍生物使用鞭毛，而UPEC菌株使用鞭毛。使用这三种类型的菌株的代表，我们表现出的差异，在营养和途径的要求，表面运动相对于葡萄糖浓度，糖酵解途径利用，产乙酸，三羧酸（TCA）循环。此外，葡萄糖控制的鞭毛合成显示为NPEC菌株，但不为高运动性NPEC变体或UPEC菌株。对表面运动的要求可能反映了菌株之间能量代谢途径和调节的主要代谢差异。重要提示尿路感染（UTI）是最常见的细菌感染之一，由于复发和抗生素耐药性，尿路感染给医疗保健系统带来了越来越大的负担（，）。最常见的泌尿系病原体是大肠杆菌。大肠杆菌（，），其引起约80 - 90%的社区获得性UTI和40 - 50%的医院获得性UTI。毒力需要皮利和鞭毛，并且任何一个附属物都可以有助于表面运动，尽管尿路致病性大肠杆菌的表面运动性。大肠杆菌没有被检测到。我们发现不同的附属物，营养物质和途径的要求，表面运动的非致病性大肠杆菌。大肠杆菌实验室菌株和尿路致病性E.大肠杆菌菌株。我们认为这些差异是能量代谢途径和调节差异的结果。
Uropathogenic Escherichia coli (UPEC) is the causative pathogen for most uncomplicated urinary tract infections (UTIs). Motility is likely to contribute to these infections, and E. coli possesses flagellum-dependent swimming motility, flagellum-dependent surface motility (often called swarming), and the recently observed pilus-dependent surface motility. Surface motility has not been extensively studied, but for the strains that have been tested, nonpathogenic E. coli (NPEC) laboratory strains use pili, NPEC hypermotile derivatives of these laboratory strains use flagella, and UPEC strains use flagella. Using a representative of these three types of strains, we showed differences in the nutritional and pathway requirements for surface motility with respect to the glucose concentration, the glycolytic pathway utilized, acetogenesis, the tricarboxylic acid (TCA) cycle. In addition, glucose-controlled flagellum synthesis was shown for the NPEC strain but not for the hypermotile NPEC variant or the UPEC strain. The requirements for surface motility are likely to reflect major metabolic differences between strains for the pathways and regulation of energy metabolism.IMPORTANCEUrinary tract infections (UTIs) are one of the most common bacterial infections and are an increasing burden on the health care system because of recurrence and antibiotic resistance (, ). The most common uropathogen is E. coli (, ), which is responsible for about 80 to 90% of community-acquired UTIs and 40 to 50% of nosocomially acquired UTIs . Virulence requires both pili and flagella, and either appendage can contribute to surface motility, although the surface motility of uropathogenic E. coli has not been examined. We found different appendage, nutrient, and pathway requirements for the surface motility of a nonpathogenic E. coli laboratory strain and a uropathogenic E. coli strain. We propose that these differences are the result of differences in the pathways and regulation of energy metabolism.