Kinetics of unfolding the human telomeric DNA quadruplex using a PNA trap
Kinetics of unfolding the human telomeric DNA quadruplex using a PNA trap
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DOI：
10.1021/ja029149w
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发表时间：
2003-04-02
影响因子：
15
通讯作者：
Balasubramanian, S
中科院分区：
文献类型：
--
作者：
Green, JJ;Ying, LM;Balasubramanian, S
利用实时荧光共振能量转移（FRET）测量和肽核酸（PNA）陷阱，研究了由人类端粒重复形成的DNA四重体的打开动力学。研究发现，对于PNA来说，这个开放是零阶的，表明初始步骤是四重体的限速内部重排。研究了温度对四联打开速率的依赖性，估计该过程的活化能为98 +/- 8 kJ mol（-1）。
The kinetics of opening of the DNA quadruplex formed by the human telomeric repeat have been investigated using real-time fluorescence resonance energy transfer (FRET) measurements with a peptide nucleic acid (PNA) trap. It has been found that this opening is zero-order with respect to PNA, indicating that the initial step is a rate-limiting internal rearrangement of the quadruplex. A study of the temperature dependence of the rate of quadruplex opening was performed and the activation energy of the process estimated to be 98 +/- 8 kJ mol(-1).