The structural organization of the readily releasable pool of synaptic vesicles
The structural organization of the readily releasable pool of synaptic vesicles
复制标题
DOI：
10.1126/science.1094682
复制
发表时间：
2004-03-26
期刊：
影响因子：
56.9
通讯作者：
Betz, WJ
中科院分区：
文献类型：
--
作者：
Rizzoli, SO;Betz, WJ
化学突触的形态学特征是突触前神经末梢的囊泡簇。一般认为，最接近释放位点的囊泡在神经活动期间首先被募集。我们通过选择性标记“易释放”池来测试这一点，这些囊泡在生理刺激期间首先释放。容易释放的囊泡没有聚集在突触前膜附近，而是几乎随机地分散在整个囊泡簇中。因此，囊泡不是根据接近释放位点而募集的，而是以不同的方式动员的，可能是通过从簇的表面剥离。
The defining morphological feature of chemical synapses is the vesicle cluster in the presynaptic nerve terminal. It has generally been assumed that vesicles closest to release sites are recruited first during nerve activity. We tested this by selectively labeling the "readily releasable" pool, those vesicles released first during physiological stimulation. The readily releasable vesicles were not clustered close to the presynaptic membrane but instead were dispersed almost randomly throughout the vesicle cluster. Thus, vesicles are not recruited according to proximity to release sites but are mobilized differently, perhaps by being peeled from the surface of the cluster.