IMMUNOGLOBULIN HEAVY AND LIGHT CHAIN GENES REARRANGE INDEPENDENTLY AT EARLY STAGES OF B-CELL DEVELOPMENT
IMMUNOGLOBULIN HEAVY AND LIGHT CHAIN GENES REARRANGE INDEPENDENTLY AT EARLY STAGES OF B-CELL DEVELOPMENT
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DOI：
10.1016/0092-8674(93)90398-a
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发表时间：
1993-03-12
期刊：
影响因子：
64.5
通讯作者：
RAJEWSKY, K
中科院分区：
文献类型：
--
作者：
EHLICH, A;SCHAAL, S;RAJEWSKY, K
通过多色流式细胞术，小鼠 B 细胞祖细胞的区室可分为五个发育相关的部分。使用该系统并采用突变小鼠，其中 mu 链的膜外显子、lambda5 基因或 J(H) 基因座通过基因靶向失活，我们发现前 B 细胞受体复合物的表达对于从大 CD43+ 到小 CD43- 前 B 细胞阶段的转变是必要的。我们报道了大 B 细胞前体阶段免疫球蛋白重链和轻链基因重排的发生。我们表明，在早期 B 细胞祖细胞中诱导 K 轻链基因重排既不需要前 B 细胞受体复合物，也不需要重链基因座中的任何基因重排。
The compartment of mouse B cell progenitors can be resolved into five developmentally related fractions by multicolor flow cytometry. Using this system and employing mutant mice in which the membrane exon of the mu chain, the lambda5 gene, or the J(H) locus was inactivated by gene targeting, we found that expression of the pre-B cell receptor complex is necessary for the transition from the large CD43+ to the small CD43- pre-B cell stage. We report the occurrence of immunoglobulin heavy and light chain gene rearrangement at the stage of large B cell precursors. We show that neither the pre-B cell receptor complex nor any gene rearrangement in the heavy chain locus is required for the induction of K light chain gene rearrangement in early B cell progenitors.