Wireless fast-scan cyclic voltammetry measurement of histamine using WINCS - a proof-of-principle study
Wireless fast-scan cyclic voltammetry measurement of histamine using WINCS - a proof-of-principle study
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DOI：
10.1039/c2an16038b
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发表时间：
2012-01-01
期刊：
影响因子：
4.2
通讯作者：
Lee, Kendall H.
中科院分区：
文献类型：
--
作者：
Chang, Su-Youne;Jay, Taylor;Lee, Kendall H.
组胺是人们最了解的生物胺之一，但组胺能系统遍布整个大脑，并与稳态和突触可塑性等多种功能有关。毫不奇怪，它与许多疾病有关，包括最低意识状态、持续性植物人状态、癫痫、成瘾、丛集性头痛、特发性震颤和帕金森病。我们之前曾报道过，无线瞬时神经递质浓度传感（WINCS）系统可以使用快速扫描循环伏安法（FSCV）监测多巴胺、血清素和腺苷。在这里，我们展示了 WINCS 系统测量组胺的扩展功能。最佳 FSCV 波形被确定为在 0.4 和 +1.4 V 之间以 400 V s(-1) 的速率在 10 Hz 下扫描的三角波。使用这种优化的 FSCV 参数，我们发现组胺释放是由大鼠脑切片中结节乳头核的高频电刺激引起的。我们的结果表明，WINCS 系统可以提供可靠、高保真度的组胺测量，始终显示 +1.3 V 的氧化电流，这一发现可能具有重要的临床意义。
Histamine is among the most poorly understood biogenic amines, yet the histaminergic system spreads throughout the brain and has been implicated in functions as diverse as homeostasis and synaptic plasticity. Not surprisingly then, it has been linked to a number of conditions including minimally conscious state, persistent vegetative state, epilepsy, addiction, cluster headache, essential tremor, and Parkinson's disease. We have previously reported that the Wireless Instantaneous Neurotransmitter Concentration Sensing (WINCS) system can monitor dopamine, serotonin, and adenosine using fast-scan cyclic voltammetry (FSCV). Here, we demonstrate the expanded capability of the WINCS system to measure histamine. The optimal FSCV waveform was determined to be a triangle wave scanned between 0.4 and +1.4 V at a rate of 400 V s(-1) applied at 10 Hz. Using this optimized FSCV parameter, we found histamine release was induced by high frequency electrical stimulation at the tuberomammillary nucleus in rat brain slices. Our results suggest that the WINCS system can provide reliable, high fidelity measurements of histamine, consistently showing oxidative currents at +1.3 V, a finding that may have important clinical implications.