Treadmill exercise prevents learning and memory impairment in Alzheimer's disease-like pathology.
Treadmill exercise prevents learning and memory impairment in Alzheimer's disease-like pathology.
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DOI：
10.2174/1567205011310050006
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发表时间：
2013-06
影响因子：
2.1
通讯作者：
Alkadhi KA
中科院分区：
文献类型：
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作者：
Dao AT;Zagaar MA;Levine AT;Salim S;Eriksen JL;Alkadhi KA
阿尔茨海默病（AD）是一种以进行性记忆丧失为特征的神经退行性疾病。相反，越来越多的证据表明，定期锻炼对衰老相关的记忆障碍具有神经保护作用。在这项研究中，我们研究了定期运动对大鼠AD模型海马CA1区短期记忆（STM）和早期长期增强（E-LTP）损伤的预防能力（i.c.v.输注250 pmol/da β1 - 42肽）。我们对4组成年Wistar大鼠进行行为评估、体内电生理记录和免疫印迹分析：对照组、跑步机运动组（Ex）、β-淀粉样蛋白输注组（Aβ）和淀粉样蛋白输注/跑步机运动组（Ex/Aβ）。我们的研究结果表明，与所有其他组相比，abβ大鼠在桡臂水迷宫（RAWM）中出现了更多的错误，CA1区E-LTP受到抑制，这与记忆和E-LTP相关信号分子（包括钙调磷酸酶（PP2B）、脑源性神经营养因子（BDNF）和磷酸化CaMKII (p-CaMKII)）水平的有害改变有关。与对照组相比，Ex和Ex/ abβ大鼠表现出相似的行为表现和正常的E-LTP， PP2B、BDNF和p-CaMKII水平没有有害变化。我们的结论是，跑步机运动可能能够预防与AD病理相关的认知障碍。
Alzheimer’s disease (AD) is a neurodegenerative disorder that is characterized by progressive memory loss. In contrast, accumulating evidence suggests a neuroprotective role of regular exercise in aging associated memory impairment. In this study, we investigated the ability of regular exercise to prevent impairments of short-term memory (STM) and early long-term potentiation (E-LTP) in area CA1 of the hippocampus in a rat model of AD ( i.c.v. infusion of 250 pmol/day Aβ1–42 peptides). We utilized behavioral assessment, in vivo electrophysiological recording, and immunoblotting in 4 groups of adult Wistar rats: control, treadmill exercise (Ex), β-amyloid-infused (Aβ), and amyloid-infused/treadmill exercised (Ex/Aβ). Our findings indicated that Aβ rats made significantly more errors in the radial arm water maze (RAWM) compared to all other groups and exhibited suppressed E-LTP in area CA1, which correlated with deleterious alterations in the levels of memory and E-LTP-related signaling molecules including calcineurin (PP2B), brain derived-neurotrophic factor (BDNF) and phosphorylated CaMKII (p-CaMKII). Compared to controls, Ex and Ex/Aβ rats showed a similar behavioral performance and a normal E-LTP with no detrimental changes in the levels of PP2B, BDNF, and p-CaMKII. We conclude that treadmill exercise maybe able to prevent cognitive impairment associated with AD pathology.