Apoptosis and nuclear shapes in benign prostate hyperplasia and prostate adenocarcinoma: Comparison with and relation to Gleason score
Apoptosis and nuclear shapes in benign prostate hyperplasia and prostate adenocarcinoma: Comparison with and relation to Gleason score
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DOI：
10.1046/j.1442-2042.1999.06116.x
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发表时间：
1999-01-01
影响因子：
2.6
通讯作者：
Jung, TY
中科院分区：
文献类型：
--
作者：
Choi, NG;Sohn, JH;Jung, TY
提议：所以比较凋亡小体的数量和细胞核形状的差异（大小和圆度）之间的未治疗的良性前列腺增生（BPH）和未治疗的晚期前列腺腺癌（PCA），并阐明细胞凋亡数量和核形状的差异，随着恶性潜能的增加，通过Gleason评分，在PCA中。对40例BPH患者和12例PCA I（Gleason评分2-4）、14例PCA II（Gleason评分5-7）和14例PCA III（Gleason评分8-10）患者进行回顾性研究。在免疫染色上检查凋亡小体的频率（从200个细胞/高倍视野计算10个视野的平均百分比）。通过自动图像分析仪确定核形状。结果：BPH组与PCA Ⅰ组凋亡小体平均数无显著性差异，而PCA IT组和PCA Ⅲ组凋亡小体平均数显著高于BPH组。PCA III患者的凋亡小体明显多于PCA I患者。良性前列腺增生细胞核的平均核面积最小，平均核周椭圆最大。研究表明，PCA I.二.与前列腺增生患者的细胞核相比，III型细胞核的面积显着更大，形状不那么圆。PCA I患者的细胞核比PCA III患者的细胞核更小，更圆。结论：本研究清楚地表明，BPH和PCA细胞凋亡的存在，并显示越来越多的凋亡小体具有较高的细胞恶性度。PCA的细胞核随着细胞恶性程度的增加而变得更不规则、更大。与癌细胞核相比，前列腺增生细胞核更小、更圆。
Propose: So compare differences in the number of apoptotic bodies and nuclear shapes (size and roundness) between untreated benign prostate hyperplasia (BPH) and untreated advanced prostate adenocarcinoma (PCA) and to elucidate differences in apoptosis number and nuclear shapes with increasing malignant potentiality, by Gleason score, in PCA.Methods: A retrospective study was conducted on 40 patients with BPH and 12 patients with PCA I (Gleason score 2-4), 14 patients with PCA II (Gleason score 5-7) and 14 patients with PCA III (Gleason score 8-10). The frequency of apoptotic bodies (mean percentage calculated from 200 cells/high-power field over 10 fields) was examined on immunostain. Nuclear shapes were determined by an automatic image analyzer. Over 100 hyperplastic cells or cancer cells were detected by the image analyzer.Results: The mean number of apoptotic bodies in BPH and PCA I were not significantly different, but patients with PCA IT and PCA III showed significantly higher numbers of apoptotic bodies than patients with BPH. Patients with PCA III had significantly more apoptotic bodies than patients with PCA I. Benign prostate hyperplasia nuclei had the smallest mean nuclear area and the largest mean nuclear peripheral ellipse among the four groups. The study showed that PCA I. II. III nuclei had significantly larger areas and a less circular shape than nuclei from patients with BPH. Nuclei from patients with PCA I were smaller in size and rounder than nuclei from patients with PCA III.Conclusions: The present study clearly shows the presence of apoptosis in BPH and PCA and shows an increasing number of apoptotic bodies with higher cellular malignancy. The nuclear shapes in PCA were more irregular and larger in size with increasing cellular malignancy. As expected nuclei in BPH were smaller in size and rounder than those of cancer nuclei.