Crevicular fluid glutathione levels in periodontitis and the effect of non-surgical therapy
Crevicular fluid glutathione levels in periodontitis and the effect of non-surgical therapy
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DOI：
10.1111/j.1600-051x.2009.01504.x
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发表时间：
2010-01-01
影响因子：
6.7
通讯作者：
Chapple, Iain L. C.
中科院分区：
文献类型：
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作者：
Grant, Melissa M.;Brock, Gareth R.;Chapple, Iain L. C.
P>目的量化牙周炎患者治疗前龈沟液 (GCF) 中的还原型谷胱甘肽和氧化型谷胱甘肽（GSH 和 GSSG）水平（与牙周健康对照相比），并确定成功的非手术治疗是否会改变谷胱甘肽水平。材料和方法在 Periopaper (TM) 条上收集了 32 个 GCF 样本（6 个/受试者）。患有慢性牙周炎、非手术治疗前和非手术治疗后 3 个月的非吸烟者（n=20；平均年龄 43.6 岁），以及牙周健康、年龄和性别匹配的对照者（n=20）。使用带有荧光检测的反相高效液相色谱法测定 GSH 和 GSSG 水平。结果在患者 (治疗前和治疗后) 的 GCF 中检测到的 GSH (p < 0.01) 和 GSSG (p < 0.05) 浓度低于对照组，治疗没有显着影响。患者和对照组之间每 30 秒样本的数量没有差异。然而，治疗后患者每 30 秒样本的 GSSG 量有所减少 (p < 0.05)。因此，与对照组 (p=0.8) 相比，治疗增加了患者的 GSH:GSSG 比率 (p < 0.05)。结论这些数据表明 GCF 内 GSH 浓度较高，在慢性牙周炎中会受到损害。虽然治疗似乎并不能完全恢复GCF中的GSH浓度，但它确实恢复了氧化还原平衡（GSH：GSSG比率），这表明氧化还原平衡异常是继发于牙周炎症引起的氧化应激的。
P>AimTo quantify reduced and oxidized glutathione (GSH and GSSG) levels in gingival crevicular fluid (GCF) of periodontitis patients pre-therapy (versus periodontally healthy controls) and ascertain whether successful non-surgical therapy alters glutathione levels.Materials and MethodsThirty-second GCF samples (6/subject) were collected on Periopaper (TM) strips from starved, non-smokers (n=20; mean age 43.6 years) with chronic periodontitis, before and 3 months after non-surgical therapy, and periodontally healthy, age- and gender-matched controls (n=20). GSH and GSSG levels were determined using reversed-phase high-performance liquid chromatography with fluorescence detection.ResultsLower concentrations of GSH (p < 0.01) and GSSG (p < 0.05) were detected in GCF from patients (pre- and post-therapy) than controls and treatment had no significant effect. Amounts per 30-second sample did not differ between patients and controls. However, the amount of GSSG per 30-second sample decreased in patients after therapy (p < 0.05). Consequently, therapy increased the GSH:GSSG ratio (p < 0.05) in patients compared with the controls (p=0.8).ConclusionThese data demonstrate high concentrations of GSH within GCF, which are compromised in chronic periodontitis. While therapy does not appear to fully restore GSH concentrations in GCF, it does restore the redox balance (GSH:GSSG ratio), suggesting that the abnormal redox balance arises secondary to oxidative stress resulting from periodontal inflammation.